重组人骨形成蛋白-3的克隆及其在大肠杆菌中的表达  

Cloning and Expression of rhBMP 3 in Escherichia coli

作　　者：陈亚兵[1] 俞春东[1] 温龙平[1] 曾定[1] 

机构地区：[1]厦门大学肿瘤细胞工程国家专业实验室

出　　处：《生物工程学报》1997年第2期210-213,共4页Chinese Journal of Biotechnology

摘　　要：重组人骨形成蛋白３的克隆及其在大肠杆菌中的表达陈亚兵俞春东温龙平曾定（厦门大学肿瘤细胞工程国家专业实验室厦门３６１００５）骨形成蛋白（ＢｏｎｅＭｏｒｐｈｏｇｅｎｅｔｉｃＰｒｏｔｅｉｎ，ＢＭＰ）是一类能诱导异位骨及软骨形成，并在动物的发育和分化中起作...Here is shown the cloning and expression of BMP 3 cDNA in E.coli .Utilizing specific primers designed according to published sequences,a 0 3kb band was assessed in various human tissues,namely brain,liver,thymus,spleen,placenta and testis,as well as human neuroblastoma SK cells by RT PCR.The 0 3kb fragment was inserted into pCRII vector and the sequencing result identified it the expected 288 bp BMP 3 cDNA.The expression of this BMP gene fragment was acquaired by ligating it with pGEX 2T and transforming E.coli .The transformed E.coli yielded high level of BMP 3 GST fusion proteins.Furthermore,the activities of the fusion proteins was domestrated by Western blotting.

关 键 词：骨形成蛋白-3 重组 克隆 大肠杆菌 表达 

分 类 号：R392－33[医药卫生—免疫学] R394.8[医药卫生—基础医学]