反相高效液相色谱法同时测定抗脑衰胶囊中葛根素和2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量  

Simultanous RP-HPLC Determination of Puerarin and 2,3,5,4'-Tetrahydroxy-stibene-2-O-β-D-Glycoside in Kangnaoshuai Capsule

作　　者：郭炎荣[1] 路玫[1] 

机构地区：[1]广西壮族自治区人民医院,广西南宁530021

出　　处：《时珍国医国药》2008年第1期110-111,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立抗脑衰胶囊中葛根素和2，3，5，4：四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定方法。方法采用反相高效液相色谱法，色谱柱为Nova-Pak C18（150mm×3．9mm，4μm），流速0．8ml·ml^-1，乙腈：水（20：80）为流动相．检测波长为320nm。结果葛根素的线性范围为0．3056—1．528mg·ml^-1，r=0．9997，平均回收率为98．15％．RSD=1．20％（n=6）；2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的线性范围为1．024～8．192μg．ml^-1，r=0．9995，平均回收率为97．46％，RSD=2．12％（n=6）。结论该方法简便、快速、准确，适合该胶囊中葛根素和2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定。Objective To establish a method for the determination of puerarin and 2,3,5,4 ′- Tetrahydroxy -stibene-2- O-β-D- glycoside in Kangnaoshuai capsule. Methods RP-HPLC was conducted on a waters Nova - PakC18 column（4 μm, 150 mm ×3.9 mm） with acetonitrile -water（20：80）, the flow rate was 0.8 ml · ml^- 1, the detection wavelength was 320nm. Results The linear range was 0. 3056 - 1. 528 mg · ml^ - 1 （ r = 0.999 7 ）, the average recovery was 98.15% , RSD = 1.20% （ n = 6） for puerarin and the linear range was 1. 024 - 8. 192 μg · ml ^- 1 （ r = 0. 999 5 ） , the average recovery was 97.46% , RSD = 2.12% （ n = 6） for 2,3, 5,4′- Tetrabydroxy - stibene -2- O-β-D- glycoside. Conclusion The method is simple, sensitive, accurate and suitable for the determination of puerarin and 2,3,5,4 ′- Tetrabydroxy - stibene -2- O-β-D- glycoside in Kangnaoshuai capsule.

关 键 词：反相高效液相色谱法 葛根素 2 3 5 ′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]