脂蛋白脂酶基因PvuⅡ酶切位点多态性与高脂血症的关系  

DNA polymorphism of pvuII sites in the lipoprotein lipase gene in patients with hyperlipoidemia

作　　者：崔晓栋[1] 张晓芸[1] 姜妙娜[2] 宋林萱[3] 葛光岩[4] 贾玉杰[2] 

机构地区：[1]山东省潍坊医学院病理生理教研室,潍坊261041 [2]辽宁省大连医科大学病理生理教研室 [3]大连市第二人民医院内分泌科 [4]大连市第三人民医院,大连116027

出　　处：《江西医药》2008年第1期16-19,共4页Jiangxi Medical Journal

摘　　要：目的探讨脂蛋白脂酶基因第6内含子PvuⅡ酶切位点多态性与高脂血症发生之间的关联性。方法采用聚合酶链反应及限制性片段长度多态性方法对辽宁大连地区144例高脂血症患者及173例血脂正常者脂蛋白脂酶基因PvuⅡ酶切位点多态性及其与血脂、载脂蛋白水平的关联进行对照研究。结果高脂血症组P+等位基因频率较对照组增加(0.79vs0.64,P<0.01);而P-等位基因频率则明显低于对照组(0.21vs0.36,P<0.01)。高脂血症组P+P+基因型者血浆甘油三酯水平明显高于P+P-和P-P-(P<0.01);血浆总胆固醇水平、LDL也高于P+P-和P-P-(P<0.05),而HDL-C水平则降低。结论脂蛋白脂酶基因内含子PvuⅡ酶切位点多态性与高脂血症有一定关联。Objective To investigate the fiequeney of variant of lipoprotein lipase （LPL） gene,PvuⅡ at intron 6, and its relation to hyperlipoidemia.Methods PCR-RFLP method was used to determine the DNA polymorphism of 6th intro, at LPL gene in 144 hyperlipoidemia patients and 173 controls.Results The P＋ allelie frequency of hyperlipoidemia group was higher than that of control group （0.79 vs 0.64,P〈0.01）.But the P- allelie frequency of hyperlipoidemia group was significantly lower （0.21 vs 0.36,P〈0.01）.The plasma triglycerides （TG） level of P＋P＋ genotype was significantly higher than that of P＋P- and P-P-genotypes （P〈0.01）in hyperlipoidemia group; the plasma TC level and TG, LDL-C ratio were higher than those of P＋P- and P-P- genotypes （P〈0.05）, while the HDL-C was lower.Conclusion The LPL- PvuⅡ -RFLP is significantly associated with hyperlipoidemia.

关 键 词：2型糖尿病 脂蛋白脂酶 基因多态性 高脂血症 

分 类 号：R587.1[医药卫生—内分泌]