辣椒Ca NPR1-RNAi表达载体的构建及其对辣椒的转化  

Construction of pepper CaNPR1-RNAi expression vector and its transformation to pepper

作　　者：罗欢[1] 段承杰[1] 陈保善[1] 唐纪良[1] 冯家勋[1] 

机构地区：[1]广西亚热带生物资源保护利用重点实验室

出　　处：《广西植物》2008年第1期107-112,99,共7页Guihaia

基　　金：广西创新能力建设项目(0322027-2);教育部高等学校骨干教师资助计划([2000]65)~~

摘　　要：从辣椒中克隆到一个与拟南芥系统获得抗性正调节基因NPR1同源的CaNPR1基因的全长cDNA。辣椒CaNPR1基因与拟南芥NPR1基因在mRNA水平上同源性为62.9%,两者的编码产物一致性为49·7%,相似性为65.7%。为鉴定该基因的功能,构建了一个以CaNPR1基因为靶基因的RNA干涉辣椒表达载体pCaNPR1-RNAi,并用根癌农杆菌介导法转化辣椒桂研五号,共获得了6株卡那霉素抗性再生苗,经Southern杂交证实,这些再生苗均为转基因植株,为进一步鉴定该基因的功能奠定了基础。Full-length cDNA of CaNPR1, a homologous gene of the positive regulatory gene NPR1 in systemic acquired resistance of Arabidopsis thaliana,was cloned from pepper（Capsicum annuum）. CaNPR1 and NPR1 share 62.9% homology at mRNA level and their encoded products have 49. 7% identity and 65.7% similarity. In order to identify the function of CaNPR1 in SAR of pepper, an expression vector pCaNPR1-RNAi for silencing CaNPR1 in pepper was constructed. Pepper cultivar Guiyan5 was transformed with pCaNPR1-RNAi by Agrobacterium tumefaciens-mediated transformation. Six kanamycin-resistant transgenic seedlings were obtained. Southern analysis confirmed that all the six seedlings carried the introduced transgene.

关 键 词：CaNPR1基因 克隆 RNA干涉 辣椒遗传转化 

分 类 号：Q943[生物学—植物学]