^41Ca标记骨质疏松大鼠体内钙库法评价市售钙剂钙吸收率的研究  

STUDY ON CALCIUM ABSORPTIVITY IN CALCIUM SUPPLEMENTS EVALUATED BY ^(41)CA LABELED CALCIUM POOL OF OSTEOPOROTIC RATS

作　　者：米生权[1] 赵晓红[1] 姜山[2] 何明[2] 李世红[2] 李冰[1] 

机构地区：[1]北京联合大学应用文理学院生物学系,北京100083 [2]中国原子能科学研究院核物理研究所,北京102413

出　　处：《营养学报》2008年第1期39-42,47,共5页Acta Nutrimenta Sinica

基　　金：北京市教育委员会科技发展计划面上项目(No.KM20041141006);国家自然科学基金(No.10405036)

摘　　要：目的建立评价市售钙剂的钙吸收率方法。方法用去卵巢大鼠建立骨质疏松模型,41Ca标记大鼠体内钙库,原子吸收检测粪钙和血钙;以加速器质谱检测粪钙和血钙41Ca峰度。结果大鼠对该市售钙剂的表观吸收率为59.15%,净吸收率为69.18%,其中粪便内源钙占24.56%。结论41Ca标记体内钙库可得出净钙吸收率,且不受所评价的钙剂中钙的化学形式和种类的影响。此法能直接应用于市售钙剂钙吸收率的评价。Objective To establish a evaluation method for absorptivity of marketed calcium supplements. Method The model of osteoporotic rats established by ovariectomy was labeled with ^41Ca. The total calcium in biological samples was measured by flame atomic absorption spectrometry （FAAS） after microwave-digestion and ^41Ca/^40Ca was detected by accelerator mass spectrometry （AMS） by using CaF2 as target in Beijing HI-13 AMS system. Results The apparent absorptivity of calcium supplements was 59.15% , the net absorptivity was 69.18% and endogenous calcium of total fecal calcium was 24,56% in the osteoporotic ovariectomic rats. Conclusion Using 41Ca labeled calcium pool in vivo could obtain the net calcium absorptivity without extrinsic labeling. Therefore, the method was not affected by the chemical structure and sorts of calcium supplements ,and might be used in evaluating absorptivity of marketed calcium supplements： [ACTA NUTRIMENTA SINICA, 2008,30（1）： 39-42, 47]

关 键 词：补钙制剂 生物利用率 表观吸收率 净钙吸收率 骨质疏松 同位素技术 ^41Ca 

分 类 号：R96[医药卫生—药理学] R580[医药卫生—药学]