视黄酸对肠道树突状细胞成熟分化及其功能的影响  

EFFECT OF RETINOIC ACID ON DIFFERENTIATION,MATURATION AND FUNCTION OF INTESTINAL DENDRITIC CELL

作　　者：董萍[1] 王卫平[1] 杨毅[1] 

机构地区：[1]复旦大学附属儿科医院,上海200032

出　　处：《营养学报》2008年第1期43-47,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30371210)

摘　　要：目的研究视黄酸对粘膜树突状细胞(DC)的成熟分化及其功能的影响,从免疫应答的初始环节探讨维生素A影响肠粘膜免疫的作用及其机制。方法大鼠肠粘膜体外培养,加入全反式视黄酸(RA)和/或视黄酸受体α(RARα)拮抗剂(Ro41-5253),于培养24h、48h收获后,1.采用流式细胞仪检测大鼠DC表面分化标志OX62、OX6和CD86的荧光强度,观察RA对DC成熟分化的影响;2.抽提RNA,采用荧光定量PCR法测定细胞因子和RARαmRNA表达水平,分析其对粘膜免疫的作用及途径。结果体外培养中加入RA,DC数目未受影响,但可明显促进DC成熟,并且RARαmRNA水平上调,该作用于培养24h显著。RA还可下调Th1细胞因子IL-12和IFN-γmRNA表达,对Th2细胞因子IL-4的产生无明显影响,可促进调节性细胞因子IL-10的mRNA表达。Ro41-5253可逆转RA的上述作用。结论对DC的调节作用可能是维生素A影响肠道粘膜免疫的重要机制之一,RARα参与介导了RA的调节作用。Objective To investigate the influence of retinoic acid （RA） in differentiation, maturation and function of intestinal dendritic cells （DC）, and probe into the effect of Vitamin A （VA） on intestinal mucosal immunity and pathway. Method Rat intestinal mucosa was cultured in vitro, supplemented with all-trans RA and/or retinoid acid receptor α （RARα） antagonist （Ro 41-5253）. After cultured for 24h and 48h, the surface markers OX62, OX6 and CD86 on DCs were detected by flow cytometry, to observe the effect of RA on differentiation and maturation of DC. The cytokines and RARα were measured in mRNA levels by RT-PCR to analyze the influence of RA on mucosal immunity and the pathway. Results RA promoted the maturation of DC in mucosa cultured in vitro, and up-regulated RARα mRNA levels, the changes were marked at 24h. Moreover, when RA was present in the culture, IL-12 and IFN-γ （Thl cytokine） mRNA were reduced, and IL-10 was increased significantly compared with control. However, the actions of RA above can be inversed by Ro 41-5253. Conclusion The modulation of RA on DC could be one of the important mechanisms that VA influences intestinal mucosal immunity. RARa participates in the regulation of RA on DC. [ACTA NUTRIMENTA SINICA, 2008, 30（1）： 43-47]

关 键 词：视黄酸 视黄酸受体 树突状细胞 细胞因子 粘膜免疫 

分 类 号：R392[医药卫生—免疫学]