抑制性受体LAIR-1表达在移植物排斥反应中的意义  

Role of expression of an inhibitory receptor LAIR-1 in graft rejection

作　　者：李州利[1] 石炳毅[1] 蔡明[1] 洪宝发[2] 张赟[3] 徐竹蔚[3] 金伯泉[3] 

机构地区：[1]解放军总医院第二附属医院全军器官移植中心,北京100091 [2]解放军总医院泌尿外科 [3]第四军医大学免疫教研室

出　　处：《解放军医学杂志》2008年第1期15-16,共2页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学“十五”计划重大项目基金资助课题(01Z097)

摘　　要：目的探讨可溶性LAIR-1(sLAIR-1)表达与移植物排斥反应的关系。方法应用双单克隆抗体夹心ELISA方法检测20例健康志愿者及162例肝、肾移植术后患者血清sLAIR-1水平,并进行分析比较。结果20例健康志愿者和98例移植物功能正常患者分别作为对照组,其血清sLAIR-1均有低水平的表达,分别为4.3±2.3μg/L和6.3±3.7μg/L,二者之间无显著差异(P>0.05)。6例移植肝急性排斥、20例移植肾急性排斥以及5例移植肾失功的患者血清中sLAIR-1表达水平显著升高,分别为47.2±25.9、36.3±14.7和28.8±9.4μg/L,与健康志愿者和移植物功能正常组之间有显著差异(P<0.01)。在1例移植肝重度排斥患者中,血清sLAIR-1水平高达117.3μg/L。5例移植肝慢性排斥、27例移植肾慢性排斥以及6例等待肾移植的透析患者血清sLAIR-1的表达水平分别为16.1±6.4、13.1±5.5和11.2±4.6μg/L,亦明显高于健康志愿者和移植物功能正常组(P<0.01)。结论sLAIR-1在发生移植物排斥反应的患者血清中有较高水平的表达,有望成为移植物排斥反应的监测指标。Objective Based on the detection of soluble leukocyte-associated immunoglobulin-like recepter-1 （sLAIR-1） in the serum of the recipient after transplantation, the role of sLAIR-1 in graft rejection was analyzed. Methods Serum sLAIR-1 level was determined by double mAb sandwich enzyme linked immunosorbent assay （ELISA） in 20 healthy volunteers and 162 patients of liver or kidney transplantation, and the results were analyzed and compared. Results In the healthy volunteers and 98 recipients with normal graft function, the sLAIR-1 were detected at the low levels of 4. 3552.3gg/L and 6.3±3. 7μg/L, which showed no significant difference （P=0. 054）. In the 6 cases of acute rejection of liver transplantation, 20 cases of acute rejection of renal transplantation, and 5 cases of graft loss, serum sLAIR-1 was found to be increased remarkably to high levels of 47. 2±35. 9, 36.3±14. 7 and 28.8±19.4μg/L, and they had significant differences compared with that of the healthy volunteers and with the recipients with normal graft function （P〈0.01）. It was even elevated to 117. 3μg/L in a case of severe liver rejection. Meanwhile, in the serum of 5 cases of liver chronic rejection, 27 cases of kidney chronic re- jection and another 6 cases who received no transplantation and under hemo-dialysis treatment, the levels of sLAIR-1 were 16. 1±11. 4, 13. 1±9.5 and 11.2±6.5μg/L respectively, and the velues were significantly higher than that of the healthy volunteers and of the recipients with normal graft function （P〈0.01）. Conclusions sLAIR 1 was detected at high level in the recipients suffering from acute or chronic rejection of the graft, and it might be a promising index of rejection after organ transplantation.

关 键 词：酶联免疫吸附测定 白细胞相关免疫球蛋白样受体1 移植物排斥 

分 类 号：R617[医药卫生—外科学]