腹透液对大鼠腹膜间皮细胞水通道蛋白1表达的影响  

Effects of peritoneal dialysate on the expression of aquaporin-1 in rat peritoneal mesothelial cells

作　　者：陈生晓[1] 任昊[2] 刘郑荣[2] 刘宏发[2] 林晓明[1] 

机构地区：[1]解放军第187医院肾内科,海口571159 [2]南方医科大学南方医院肾内科

出　　处：《解放军医学杂志》2008年第1期72-74,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题(30370665)

摘　　要：目的探讨腹透液对大鼠腹膜间皮细胞水通道蛋白1(AQP1)表达的影响。方法将体外培养的大鼠腹膜间皮细胞分成4组,分别用含10%胎牛血清的1640培养液(阴性对照组)和3种不同腹透液(分别含4.25%葡萄糖、4.25%甘露醇、1.5%葡萄糖)刺激3h,采用间接免疫荧光标记法、流式细胞仪检测上述不同腹透液刺激后AQP1的表达。结果4.25%葡萄糖腹透液组和4.25%甘露醇腹透液组腹膜间皮细胞AQP1表达显著大于阴性对照组和1.5%葡萄糖腹透液组(P<0.01);4.25%葡萄糖腹透液组腹膜间皮细胞AQP1表达显著大于4.25%甘露醇腹透液组(P<0.01);1.5%葡萄糖腹透液组腹膜间皮细胞AQP1表达与阴性对照组相比无显著性差异(P>0.05)。结论高糖腹透液可以促进腹膜间皮细胞表面AQP1的表达,糖本身或糖降解产物可能是促进其表达的独立因素。Objective To discuss the effects of peritoneal dialysate （PDS） on the expression of aquaporin-1 in rat peritoneal mesothelial cells （RPMCs）. Methods The in vitro cultured RPMCs were divided randomly into 4 groups, and stimulated respectively with 1640 culture medium containing 10% fetal calf serum （negative control）, and three kinds of PDS （containing respectively 4. 25% glucose, 4. 25% rnannitol or 1. 5% glucose） for 3 hours. Indirect immunofluorescence assay （IFA） and flow cytometry （FCM）were employed to de tect the expression of aquaporin-1 in RPMCs stimulated by different kind of PDS as above. Results Compared with negative control and the group with PDS containing 1.5% glucose, the expression of aquaporin-1 on the mesothelial cells of the group with PDS containing 4. 25% glucose and that containing 4. 25% mannitol was significantly up-regulated （ P〈0. 01）. Higher expression of aquaporin-1 was found in the group with PDS containing 4. 25 % glucose compared with that in the group with PDS containing 4. 250% rnannitol （P〈0. 01）. No significant differences on the expressions of aquaporin-1 were found between the negative control and the group with PDS containing 1.5% glucose （ P〉0. 05）. Conclusion PDS with higher osmotic pressure （OP） can enhance the expression of aquaporin-1 in RPMCs. For PDS with same OP （PDS containing 4. 25% glucose or PDS containing 4. 25% mannitol）, those containing glucose can enhance the expres sion of aquaporin-1 more effectively than that PDS containing rnannitol. This result indicates that glucose or its degradation products may be the independent factors in enhancing the expression of aquaporin-1 of RPMCs.

关 键 词：透析液 腹膜透析 腹膜间皮细胞 水通道蛋白 

分 类 号：R692.5[医药卫生—泌尿科学]