有机农药活性污泥中表面活性剂产生菌的筛选及鉴定  

Screening and Identification of Biosuractant-producing Bacteria from Organic Pesticides Activated Sludge

作　　者：张林达[1] 刘红玉[1] 鲁双庆[2] 贾彩云[1] 刘臻[2] 

机构地区：[1]湖南大学环境科学与工程学院,湖南长沙410082 [2]长沙大学,湖南长沙410002

出　　处：《生物技术》2008年第1期32-34,共3页Biotechnology

基　　金：国家高技术研究发展计划(863)项目资助(Supported by the High-Tech Research and Development Programof China(863)under Grant No.2004AA649370);湖南省自然科学基金项目资助(Hunan Provincial Natural Science Foundation of China under Grant No.05JJ30021)

摘　　要：目的:筛选表面活性剂产生菌,用于降解有机农药,提高氧化塘处理效率。方法:以液体石蜡为惟一碳源进行选择性培养,通过测定发酵液的排油活性和表面张力,对有机农药厂的活性污泥进行产生物表面活性剂细菌筛选,并对筛得的细菌进行形态学、生理生化及16SrDNA试验和鉴定。结果:筛选出3株产生物表面活性剂的细菌,经鉴定均属于不动杆菌属。结论:证实了有机农药活性污泥中存在表面活性剂的产生菌,对有机农药降解存在助溶作用。Objective： Biosuractam - producing bacteria was screened to improve more conductive to microbial organic pesticide absorption and degradation. Methods： Using liquid paraffin as the sole carbon source, the biosurfactant- producing bacteria have been screened, and then were identified by the method of morphological, physiological and biochemical assay, as well as 16S rDNA sequence analysis. Results： the 3 biosurfactant- producing bacteria were Acinetobacter sp. in the organic pesticide activated sludge. Conclusion： the experiment proved the imagination that there are biosurfactant- producing bacteria in organic pesticides activated sludge and the possibility that the biosurfactant- producing bacteria play an important roll in the solubilization of organic pesticides.

关 键 词：生物表面活性剂 细菌 有机农药 活性污泥 菌种鉴定 

分 类 号：Q93-331[生物学—微生物学]