缓激肽B1受体在严重烫伤大鼠内脏血管通透性变化中的作用  

Role of bradykinin B1 receptor in organic vascular permeability following severe burn

作　　者：赵明月[1] 黄跃生[1] 张琼[1] 王广[1] 阮兢[1] 雷泽源[1] 褚志刚[1] 向飞[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2008年第4期284-287,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划项目(“973”项目)(2005CB522601);全军医学科研“十一五”计划专项课题(06Z033)~~

摘　　要：目的探讨严重烫伤大鼠内脏血管通透性的变化及缓激肽B1受体在其过程中的作用。方法成年SD大鼠90只,体质量250～300g,分为正常对照组(N组)、假烫伤组(S组)、单纯烫伤组(B组)、假烫伤+缓激肽B1受体特异性抑制剂组(SI组)和烫伤+缓激肽B1受体特异性抑制剂组(BI组)。S组仅补液不烫伤,B组和BI组大鼠均造成30%Ⅲ度烫伤并补液后,分别于伤后0、3、6、12、24h和48h取肺、心、肾、小肠,通过伊文思蓝(Evan’sBlue,EB)法测定各脏器血管通透性变化。结果与S组比较,B组大鼠各脏器组织中EB含量显著增加,在伤后3h达高峰,24h内均处于较高水平,伤后48h内基本恢复到正常水平;静脉注射缓激肽B1受体特异性抑制剂后,BI组与各时相点单纯烫伤组比较,各脏器EB含量显著降低。结论缓激肽B1受体特异性抑制剂可有效降低严重烫伤大鼠伤后主要脏器血管通透性。Objective To investigate the changes of organic vascular permeability after severe burn in rats and determine if the selective bradykinin receptor 1 antagonist affects the vascular permeability. Methods Ninety rats were divided into 5 groups： normal group （group N）, sham burn group （group S）, sham burn plus bradykinin receptor 1 antagonist （BR1A, des Argg-ArgS-BK） group （group SI）, burn group （group B）, and burn plus BR1A group （group BI）. BR1A （1 mol/kg） dissolved in normal saline to 1 mol/L was applied intravenously 20 rain before the organ samples were taken. Evan＇ s blue （EB） was injected 10 min before preparation of myocardium from ventricle, lung, kidney and intestinal tissues in dimethylformamide 0, 3,6, 12, 24, 48 h after burn. The content of dissolved EB was determined by UV spectrophotometer. Results In comparison with group S, the content of EB in all organ tissues in the group B increased significantly, peaked 3 h after burn, kept at the high level in 24 h, and declined close to normal levels 48 h after burn. The content of EB was lower in all organ tissues in group BI than that in group B. Conclusion The selective inhibitor of bradykinin B1 receptor apparently inhibits the increase of vasopermeability.

关 键 词：烫伤 血管通透性 缓激肽B1受体 缓激肽B1受体特异性抑制剂 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学] R329.27[医药卫生—基础医学]