脂多糖结合蛋白的B细胞抗原表位预测及其效应初步分析  

Prediction of B-cell antigen epitope on lipopolysaccharide-binding protein

作　　者：冯起甲[1] 徐智[1] 吴国明[1] 胡川闽[2] 徐顺贵[1] 陈维中[1] 郭芮伶[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室,重庆400037 [2]第三军医大学医学检验系临床生物化学教研室,重庆400038

出　　处：《第三军医大学学报》2008年第4期292-294,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30500230)~~

摘　　要：目的预测人脂多糖结合蛋白(LBP)的B细胞抗原表位。方法采用生物信息软件和互联网服务器,预测LBP的二级结构和分析LBP表面特性(亲水性、可塑性、可及性和抗原性),再计算平均抗原指数(AI),预测LBP的B细胞抗原表位。结果LBP存在多个潜在的抗原表位,247～260序列(FKGEIFHRNHRSPV)的AI(0.0407)明显高于其他片段,370～379序列(LPSSSKEPVF)和303～325序列(ITDDMIPPDSNIRLTTKSFRPFV)的AI分别是0.0336和0.0295。247～260序列是LBP潜在的B细胞优势抗原表位。结论应用多参数预测到LBP的B细胞抗原表位。Objective To predict the B-cell epitope for lipopolysaccharide-binding protein （LBP） antigen. Methods Using biotic software and network servers, the secondary structure of LBP was predicted, and the surface properties of LBP, such as hydrophilicity, flexibility, accessibility and antigenicity, were analyzed. Then the average antigen index （AI） was calculated by using Wu＇ s method. Results Many distinct antigenic epitopes on LBP were identified ： AI of sequence 247 - 260 （FKGEIFHRNHRSPV） on LBP was 0.040 7, significantly higher than that of other sequences. AI of sequence 370 - 379 （LPSSSKEPVF） and sequence 303 - 325 （ITDDMIPPDSNIRLTYKSFRPFV） was 0.033 6 and 0.029 5, respectively. Sequence 247 - 260 was the prevalent antigen epitope of B-cell on LBP. Conclusion The B-cell epitopes of LBP is predicted successfully.

关 键 词：LBP抗原 表位 B细胞 生物信息学 

分 类 号：R318.04[医药卫生—生物医学工程] R392.1[医药卫生—基础医学]