猫运动皮层神经元和S100、GFAP阳性细胞的年龄相关性变化  

Age-Related Changes in Neurons and S100-, GFAP-Immunoreactive Cells in the Motor Cortex of Cats

作　　者：申林[1] 孙庆艳[1] 考传超[1] 华田苗[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《Zoological Research》2008年第1期56-62,共7页动物学研究（英文）

基　　金：安徽省自然科学基金(070413138);安徽省高校重点实验室科研基金;安徽师范大学博士启动基金资助

摘　　要：比较了青、老年猫运动皮层神经元与S100、GFAP免疫阳性胶质细胞的形态学变化,并探讨其与衰老过程中运动功能衰退的关系。采用Nissl染色显示青、老年猫运动皮层分层结构和神经元。免疫组织化学方法(SABC法)显示青、老年猫运动皮层S100免疫反应阳性(S100-immunoreactive,S100-IR)细胞及胶质纤维酸性蛋白免疫反应阳性(GFAP-immunoreactive,GFAP-IR)细胞。在Olympus显微镜下,用Moitcam5000数码成像与分析系统计数运动皮层各层神经元、S100-IR细胞及GFAP-IR细胞的数量,并随机抽样测量S100-IR、GFAP-IR细胞的胞体直径。与青年猫相比,老年猫运动皮层Ⅴ、Ⅵ层神经元密度显著下降(P<0.01),老年猫运动皮层中S100-IR和GFAP-IR细胞密度与胞体直径均显著增加(P<0.01),且细胞的免疫阳性反应较强。研究结果表明,猫运动皮层的神经元密度在衰老过程中Ⅴ、Ⅵ层神经元密度显著下降,有可能会降低老年个体运动皮层对运动的调控能力;随着衰老、运动皮层的星形胶质细胞出现明显的反应性活化与增生,这对维持大脑运动皮层神经元的活性和神经元之间的通讯联系,从而延缓老年性运动功能衰退具有重要意义。Morphological changes of neurons and S 100-,GFAP-immunoreactive cells in the motor cortex of young adult and older cats were comparatively investigated, and the correlation of these changes and motor function degradation during senescence was discussed. Nissl staining was applied to show cortical layers and neurons. Immunohistochemical method was employed to exhibit S100-immunoreactive （S100-IR） and GFAP-immunoreactive （GFAP-IR） cells. Under an Olympus microscope, Moitcam 5000 Digital Image Acquisition and Analysis System was used to statistically count the number of total cortical neurons, GFAP-IR cells and S100-IR cells in each cortical layer, and the cell-body diameter of GFAP-IR cells and S 100-IR cells were randomly sampled and measured. The density of total neurons in layer Ⅴ and Ⅵ of the motor cortex showed a significant decrease in older cats （P〈0.01）. Furthermore, the density and the cell-body diameter of GFAP-IR and S100-IR cells in the motor cortex of old cats were significantly increased when compared to that in young adults （P〈0.01）. In addition, the S 100 and GFAP immunoreactivity in the motor cortex of older cats was stronger than in younger ones. The density of total neurons in layer Ⅴ and Ⅵ of the motor cortex was significantly reduced during aging, which might lead to a decreased motion mediation capacity of the motor cortex in older individuals Moreover, with age, astrocytes in the motor cortex were significantly activated and increased, which is of great significance in maintaining normal neuronal activities, signalling between neurons and therefore slowing down age-dependent motor function degradation.

关 键 词：运动皮层 星形胶质细胞 S100蛋白 胶质纤维酸性蛋白 年龄相关变化 

分 类 号：Q42[生物学—神经生物学]