鸡马立克氏病疫苗荧光抗体染色阳性细胞数与蚀斑数线性回归(FAC^+ PR)方法的建立及应用  

Establishment and Application of Linear Regression between Fluorescent Antibody Staining Positive Cells and Plaques Forming Unit of Marek's Disease Vaccine

作　　者：姜国华[1] 胡小元[1] 张岐蜀[1] 潘春刚[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《中国兽药杂志》2008年第2期23-26,共4页Chinese Journal of Veterinary Drug

摘　　要：为解决鸡马立克氏病(MD)疫苗,尤其是MD多价疫苗在生产中疫苗配制环节上的盲目性,本试验建立了MDCVI988/Rispens/B5疫苗荧光抗体染色阳性细胞数(FAC+/mL)与疫苗蚀斑数(PFU/mL)两个数量化指标的线性关系(FAC+PR)。统计学检验证明,在此双变量下所建立的线性回归能客观反映该疫苗FAC+细胞数与疫苗PFU数之间的相互关系。将FAC+PR方法运用于MD疫苗的实验室制备,结果理论PFU/mL与实际PFU/mL的平均误差仅为5.4%。由此可见,采用这种方法可有效、准确估计出疫苗毒液中的PFU含量,并可以此为标准进行疫苗的配制。To solve the confecting blindness of Marek＇ s disease （MD） vaccine in MD vaccine production, especially for MD polyvalent vaccines, linear regression was established between fluorescent antibody staining positive cells （FAC^＋/mL） and plaques forming unit （PFU/mL） in this experiment. It has been proved that the established linear regression could objectively reflect the relationship between MD vaccine FAC^＋/mL with MD vaccine PFU/mL each other by statistical tests. The results showed that the error of theoretical PFU/mL numbers to compared actual average PFU/mL numbers was only 5.4% when the FAC^＋ PR had been used in MD vaccine confecting in laboratory. This results shows that the FAC^＋ PR could be effectively used and accurately estimated the PFU numbers of MD vaccines, and could be used as a standard method for MD vaccine confecting.

关 键 词：鸡马立克氏病 疫苗 荧光抗体染色阳性细胞数 蚀斑数 

分 类 号：S859.797[农业科学—临床兽医学]