王锦蛇(Elaphe carinata) Sox基因保守区的克隆及测序  

Cloning and Sequencing of the Sox Genes in Elaphe Carinata

作　　者：杨超[1] 杨传秀[2] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]皖南医学院生理教研室,安徽芜湖241001

出　　处：《安徽师范大学学报（自然科学版）》2008年第1期53-56,共4页Journal of Anhui Normal University(Natural Science)

基　　金：安徽省自然科学基金(070413140);安徽省教委自然科学基金(2003kj164)

摘　　要：参照人SRY基因HMG-box保守区序列设计一对兼并引物,PCR扩增了王锦蛇的Sox基因,采用SSCP技术筛选阳性克隆,并对其进行了测序.结果在雌雄个体中共筛选出4个Sox基因,其中一个为雌性独有,显示出性别差异性;4个Sox基因DNA序列及编码的氨基酸序列与人相应SOX基因的相似性分别为91%、91%、92%、91%和96%、98%、96%、96%,显示出高度的保守性.实验结果为王锦蛇的性别决定机制研究提供了分子资料.According to the HMG-box sequence of human SRY , a pair of degenerate primers were designed and used to amply the Sox gene of Elaphe carinata using PCR technique. An approximately 220bp DNA segment were obtained from genome DNA of male and female. The PCR products were cloned using pGEM-T vector. The positive clony was identified by SSCP and the sequence was examined by automatic sequencing technique. We got 4 different Sox genes in 460 positive clones, among them, one was amplified only from female body. The result indicated that there are different between male and female. The 4 Sox genes showed high homology to Human SOX genes, and the identities to human SOX1, SOX4, SOX11, SOX22 genes in the DNA sequence and the amino acid sequence are 91% ,91% ,92% ,91% and 96% ,98% ,96% ,96%, respectively. It might be concluded that Soz gene was highly conservative in phylogenetic. These results provided molecular data for the study of Sox determining mechanism of Elaphe carinata.

关 键 词：王锦蛇 SOX基因 PCR 测序 

分 类 号：Q343[生物学—遗传学]