系统性红斑狼疮患者自体外周血纯化造血干细胞移植后免疫功能的变化  

Changs of immune function in patients with systemic lupus erythematosus after autologous peripheral blood purified stem cells transplantation

作　　者：孙乐栋[1] 孙竞[2] 曾抗[1] 孟凡义[2] 刘启发[2] 彭学标[1] 

机构地区：[1]南方医科大学南方医院皮肤科,广东广州510515 [2]南方医科大学南方医院血液科,广东广州510515

出　　处：《大连医科大学学报》2008年第1期29-32,共4页Journal of Dalian Medical University

基　　金：广东省科技计划项目基金资助(2003C32720)

摘　　要：[目的]研究自体外周血纯化造血干细胞移植对系统性红斑狼疮患者免疫功能的影响。[方法]16例接受自体外周血纯化造血干细胞移植的系统性红斑狼疮患者列入研究。应用四色荧光分析技术动态监测移植前及移植后3、6、12个月的CD3+、CD45RA+CD4+、CD45RA+CD8+、CD45RO+CD4+表达的变化情况。[结果]移植后3个月CD3+、CD45RA+CD4+、CD45RA+CD8+、CD45RO+CD4+的表达均低于移植前,其中CD3+、CD45RA+CD8+、CD45RO+CD4+的表达水平差异具有显著性意义(P<0.05)。移植后6个月CD3+、CD45RA+CD8+、CD45RO+CD4+的表达均低于移植前(P<0.05)。CD45RA+CD4+的表达水平高于移植前和移植治疗后3个月的,与移植后3个月的表达水平差异具有显著性意义(P<0.05)。移植后1年CD45RO+CD4+的表达水平低于移植前(P<0.05)。CD45RA+CD4+的表达水平高于移植前和移植后3个月和6个月,与移植后3个月的表达水平差异具有显著性意义(P<0.05)。[结论]自体外周血纯化造血干细胞移植后存在免疫功能缺陷,可持续1年之久。通过流式细胞仪检测患者外周血CD3+、CD45RA+CD4+、CD45RA+CD8+、CD45RO+CD4+表达情况可相对定量地检测患者的胸腺再生输出功能,并通过测量新生T细胞的合成来监视胸腺功能在免疫重建中的作用,为临床干细胞移植提供有力的理论依据。[ Objective] To investigate the alteration of immune function in patients after autologous peripheral blood purified stem cells transplantation. [ Methods] Sixteen patients with systemic lupus erythematosus had received autologous peripheral blood purified stem cells transplantation were enrolled in this study. Four - color flow cytometry was used to detect the express of CD^＋O3, CD45 RA^＋ CD4^＋ , CD45 RA^＋ CD8^＋ and CD45 RO^＋ CD4^＋ before transplantation and at 3,6, 12 months after transplantation. [Results] Frequence of CD3^＋ ,CD45 RA^＋ CD4^＋ , CD45 RA^＋ CD8^＋ and CD45 RO^＋ CD4^＋ in peripheral blood at 3 months after transplantation were decreased compared with that of before transplantation. At the same time, the subset of CD3^＋ , CD45 RA^＋ CD^＋ and CD45 RO^＋ CD4^＋ were lower than those of before transplantation （ P 〈 0.05 ）. At 6 months after transplantation, the CD^＋ , CD45 RA^＋ CD^＋ and CD45 RO^＋ CD4^＋ subsets were decreased compared with those of before transplantation （ P 〈 0.05 ）. CD45 RA^＋ CD4^＋ subset was higher than that of before transplantation and 3 months after transplantation. CD45 RA^＋ CD^＋ subset was increased significant at 6 months after transplantation than that of at 3 months（ P 〈 0.05 ）. Frequence of CD45 RO^＋CD4^＋ in peripheral blood at 12 months after transplantation were also decreased significant than that of before transplantation（P 〈0.05）. Frequence of CD45 RA^＋ CD4^＋ was higher than that of before transplantation, 3 and 6 months after transplantation, only increased significant than that of 3 months after transplantation （P 〈 0.05 ）. [ Conclusion ] Autologous peripheral blood purified stem cells transplantation can reduces the immune function. Measurement of the changes of CD3^＋ , CD45 RA^＋ CD4^＋ , CD45 RA^＋ CD8^＋ and CD45 RO^＋ CD4^＋ cell ratio using flow cytometry may provide insights into the molecular picture of the thymus regeneration function in association with immune re

关 键 词：造血干细胞 移植 系统性红斑狼疮 

分 类 号：R593.241[医药卫生—内科学]