不同季节元宝枫叶对FAS活性的抑制及其与总黄酮含量的相关性  

The Inhibition of FAS Activity and the Correlation with Total Flavonoids from Acer Truncatum Bunge Leaves in Different Seasons

作　　者：赵春杨[1] 赵文华[2] 张瑾峰[2] 王喆[2] 张英侠[2] 

机构地区：[1]哈尔滨商业大学药学院 [2]首都医科大学化学生物学与药学院化学生物学系

出　　处：《首都医科大学学报》2008年第1期60-63,共4页Journal of Capital Medical University

基　　金：北京市教育委员会科技发展项目(KM200410025013)~~

摘　　要：目的研究不同季节元宝枫叶提取物对动物脂肪酸合成酶(FAS)抑制能力的差异及其与叶中总黄酮含量的关系,为开发元宝枫叶提供实验依据。方法用分光光度法对不同季节的元宝枫叶对FAS的抑制能力及总黄酮的含量进行分析测定。结果不同季节的元宝枫叶对FAS的抑制能力的差异有统计学意义,其中落叶的应用价值最高,对FAS的抑制作用与总黄酮含量可能存在正相关性。结论每年11月的元宝枫叶对FAS的抑制最为显著,FAS可能是抗癌作用的潜在靶点。Objective Acer truncatum bunge is a prominent species of Acer in the hardwood forests of north China. It has been used as a Chinese folk medicine for the treatment of coronary atherosclerosis, cerebrovascular diseases and angina pectoris. Animal fatty acid synthase（E. C. 2.3. 1.85, FAS） plays an important role in fatty acid synthesis. It was reported that FAS is selectively highly expressed in certain human cancer cells, including carcinomas of the breast, prostate, colon, ovary, endometrium and lung. FAS has been suggested as a potential new therapeutic target in the treatment of cancer and FAS inhibitors may be developed into new chemotherapeutic methods. The extracts from Aceraceae leaves are found to possess a high inhibitory activity against FAS and the correlated antitumor bioactivity has also been proved in our experiment. To examine the inhibitory activity of the leaves gathered in different seasons and the correlation with the total flavonoids content, we perform the following experiment. Methods By using colorimetric methods, the content of total flavonoids in leaves of Acer truncatum bung of different seasons was determined. The assay for FAS activity which had been described previously was performed with a Shimadzu UV2550 double wavelength/double beam spectrophotometer at 37℃ by following the decrease of NADPH at 340 nm. The reaction system contained 0. 1 mol/L potassium phosphate buffer, pH 7.0 ; 1.0 mol/L EDTA ; 1.0 mmol/L DTT ; 3 mmol/L acetyl-CoA ; 10 mmol/L malonyl-CoA ; 32 mmol/L NADPH and duck liver FAS 10 microg in a total volume of 2.0 mL. Results The inhibitory ability against FAS and the content of total flavonoids exhibited a positive correlation for the extract from the leaves of May, June, July, prepared with the solvent rich in ethanol. Otherwise, the correlation would not exist. Conclusion The inhibitory ability of leaves of Acer truncatum bunge at different seasons markedly differed and the extracts from the fallen leaves possess stronger inhibitory effect on FAS. It may become

关 键 词：元宝枫叶 脂肪酸合成酶 FAS 总黄酮含量 

分 类 号：R962[医药卫生—药理学]