环氧乙烷对大鼠DNA损伤机理的研究  

A Study on the Mechanism of Damage to DNA in Rats by Ethylene Oxide

作　　者：张龄[1,2,3] 钟先玖[1,2,3] 张民 胡培蓉[1,2,3] 周元陵 范卫[1,2,3] 余龙 

机构地区：[1]上海职工医学院 [2]上海医科大学附属金山医院 [3]复旦大学遗传学研究所

出　　处：《中华预防医学杂志》1997年第4期199-201,共3页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金

摘　　要：为探讨环氧乙烷（ＥｔＯ）对ＤＮＡ分子水平的损伤作用，采用血红蛋白基因特异性的单引物，对用ＥｔＯ染毒不同时间的Ｗｉｓｔａｒ雄鼠和对照个体的肝、肾、睾丸等组织的ＤＮＡ进行半随机ＰＣＲ扩增。结果显示：在吸入染毒（８００ｍｇ／ｍ３）６～９周条件下，ＥｔＯ可引起肝、肾、睾丸等组织不同程度的ＤＮＡ损伤，其中睾丸组织ＤＮＡ损伤最为严重。DNA in the liver, kidney, testes, etc. was amplified with semi random polymerase chain reaction (PCR) in Wistar male rats exposed to ethylene oxide (EtO) for various times and in control rats with hemoglobin gene specific primer to study the damage effects of EtO on DNA molecule. Results showed that inhalation of 800 mg/m 3 of EtO for six to nine weeks could cause damage to DNA in the liver, kidney and testes, etc. to various extent, with the most serious damage to DNA in the testes.

关 键 词：环氧乙烷 DNA损伤 

分 类 号：R992[医药卫生—毒理学] R996[医药卫生—药学]