金鱼核糖体转录间隔区(ITS-1)的克隆和序列分析  

Cloning and Sequence Analysis of Ribosomal DNA Internal Transcribed Spacer 1 From Goldfish(Carassius auratus)

作　　者：牟希东[1,2] 白俊杰[1] 汪学杰[1] 罗建仁[1] 

机构地区：[1]中国水产科学院热带亚热带鱼类选育与养殖重点开放实验室,中国水产科学研究院珠江水产研究所,广州510380 [2]上海水产大学,上海200090

出　　处：《淡水渔业》2008年第1期74-76,43,共4页Freshwater Fisheries

基　　金：广东省农业攻关计划项目(2005B20301015)

摘　　要：对金鱼（Carassius auratus）核糖体转录间隔区（ITS-1）进行了克隆测序，并对ITS-1序列进行了分析。结果显示，克隆的金鱼ITS-1区序列长度为294bp，其中A、T、G、C4种碱基的含量分别为17．0％、14．6％、33．0％、35．4％；与从GenBank中检索到的12种鱼的核糖体DNA序列比较显示，其与12种鱼的ITS-1序列同源性较低，在26．0％～61．6％之间；根据金鱼与其他12种鱼的ITS-1序列的同源性构建进化树，所得的分类结果与传统的分类结果基本一致。The internal transcribed spacer 1 （ IST-1 ） from goldfish （ Carassius auratus ） was cloned, sequenced and analyzed. The result showed a sequence with total length of 294 nucleotides of ITS-1. The contents of A, T, G and C were 17. 0% , 14.6% , 33.0% , 35.4% in ITS-1 of goldfish. Comparing to ITS-1 sequences of other 12 fishes in GenBank, there were lower homologies from 26.0% to 61.6% between goldfish and other fishes. The evolution tree built from the ITS-1 homology of goldfish and other 12 fishes was consistent with that of traditional taxonomic results.

关 键 词：金鱼(Carassius auratus) 核糖体 转录间隔区1(ITS-1) 基因克隆 基因序列 

分 类 号：Q173[生物学—水生生物学]