广西三黄鸡γ-干扰素基因真核表达载体的构建及表达  

Construction and express of recombinant eukaryotic expression vector of Guangxi Sanhuang chicken interferon-gamma

作　　者：董建宝[1] 谢芝勋[1] 孙建华[1] 刘加波[1] 庞耀珊[1] 邓显文[1] 谢志勤[1] 谢丽基[1] 

机构地区：[1]广西兽医研究所,广西南宁530001

出　　处：《西南农业学报》2008年第1期167-169,共3页Southwest China Journal of Agricultural Sciences

基　　金：广西留学基金项目(桂科回0542006)

摘　　要：设计1对特异性引物,采用PCR方法从pMD18-TCHIFN-γ重组载体中扩增得到广西三黄鸡γ-干扰素基因,然后将该基因克隆入含有绿色荧光报告基团EGFP的真核表达载体pEGFP-N1中,构建成pEGFP-N1CHIFN-γ重组质粒,PCR鉴定结果和测序结果均显示,γ-干扰素基因正确地插入到真核表达载体中。使用脂质体转染法将pEGFP-N1CHIFN-γ转染Vero细胞,荧光显微镜下成功地观察到绿色荧光,说明γ-干扰素基因成功表达。One pair of specific primers was designed to clone Guangxi Sanhuang chicken interferen-γgene from the recombinant vector pMD18-CHIFN-γ. And then the interferen-γgene was inserted into eukaryotic expression vector pEGFP-N1 which included one EGFP fluorescence gene. The recombinant vecter pEGFP-N1 CHIFN-γ was proved correct by PCR and sequencing,then co-transfected into Vere cells,The fluorescence was detected by fluorescopy,so it was proved that the interferon-γwas expressed successfully.

关 键 词：鸡γ-干扰素 绿色荧光 真核表达 

分 类 号：S859.797[农业科学—临床兽医学]