狐源犬瘟热病毒泰安株(CDV-FOX-TA)的分离与鉴定  

Isolation and identification of Canine distemper virus from fox

作　　者：杨笃宝[1] 吕品[1] 胡传伟 贾赟 谢之景[1] 曹涤非[3] 任月[4] 刘海防[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]辽宁省出入境检验检疫局技术中心,辽宁大连116001 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [4]大连医科大学微生态教研室,辽宁大连116027

出　　处：《西南农业学报》2008年第1期204-207,共4页Southwest China Journal of Agricultural Sciences

基　　金：山东农业大学青年科技创新基金(23204);国家质检总局项目资助(2005IK047)

摘　　要：从具有疑似犬瘟热症状的病狐血液中分离到1株病毒,该病毒在MDCK细胞上生长良好,并能形成稳定的CPE。经电镜观察、理化特性、滴度测定、动物回归试验、RT-PCR和荧光定量RT-PCR鉴定,证实分离到的病毒为1株犬瘟热病毒强毒株,命名为CDV-FOX-TA。A virus strain was isolated from the blood of fox,which could grow well and produce stabilization cytopathic effects in MDCK cells. It was demonstrated to be a canine distemper virus by a series of systematic indentification such as electron microscope observe, morphology physicochemically, TCIDso, animals infection test, RT-PCR and real time RT-PCR. It was named CDV-FOX-TA.

关 键 词：狐狸 犬瘟热病毒 分离 鉴定 

分 类 号：S865.23[农业科学—野生动物驯养] S852.655[农业科学—畜牧兽医]