聚乙醇酸负载同种异体软骨细胞移植修复兔关节软骨缺损  

PGA Engineered Chondrocyte Homotransplantation for the Repair of Rabbit Articular Cartilage Defects

作　　者：王德春[1,2] 陈峥嵘[1,2] 宋后燕[1,2] 彭鲁英[1,2] 费琴明[1,2] 姜南春[1,2] 

机构地区：[1]上海医科大学附属中山医院骨科 [2]上海医科大学分子遗传研究室

出　　处：《中华创伤杂志》1997年第4期228-231,共4页Chinese Journal of Trauma

摘　　要：目的：应用聚乙醇酸（ＰＧＡ）负载的兔软骨细胞培养移植修复同种异体关节软骨缺损．方法：应用在生物体内可降解吸收、纤维状多孔态的ＰＧＡ作为支架行兔软骨细胞培养．培养１４天后，软骨细胞在ＰＧＡ提供的三维空间中大量分裂、增殖并合成大量软骨基质，形成ＰＧＡ－软骨细胞复合体，然后利用该复合体移植修复同种异体兔膝关节全层软骨缺损，对侧膝关节作对照．术后行大体、组织学、电镜动态观察及修复组织厚度测定．结果：ＰＧＡ在术后８周完全降解吸收，实验侧与对照侧修复组织的厚度有显著性差异（Ｐ＜０．０１）；术后１６周在实验侧可见典型的软骨组织，电镜下为成熟的软骨细胞，而对照侧为纤维组织修复．结论：应用ＰＧＡ－软骨细胞复合体移植，可修复同种异体的兔关节软骨缺损，为临床治疗关节软骨缺损奠定了基础．Aim: In an attempt to repair the defects of rabbit articular cartilage with the method of polyglycolic acid (PGA) engineered, cultured chondrocytes homotransplantaion. Methods: The biodegradable, fibrous, multiple microhole PGA was used as a vehicle for rabbit chondrocyte culture. After fourteen days of cell culture, the chondrocyte proliferated greatly and synthesized a large quantity of cartilage matrix in three dimension PGA frame. The PGA chondrocyte complex was prepared and transplanted into a large, full thickness defect in weight bearing surface of the medical femoral condyle. The contralateral knee served as a control. The macroscopic, histologic, electron microscopic evaluations and the thickness of repair tissue measurement were performed postoperatively. Results: There was no immune reaction around the grafts. The ploymer degraded completely in 8 weeks after operation. There was significant difference in the thickness of repair tissue between the grafted ones and the controls ( P <0.01). The typical cartilage tissue was formed and mature chondrocyte was observed under electron microscope 16 weeks after operation. The fibrous tissue was formed in the controls. Conclusion: The full thickness cartilage defects of rabbit has been repaired successfully with method of PGA engineered chondrocytes homotransplantation. It is highly promising for the treatment of articular cartilage defects.

关 键 词：关节软骨缺损 软骨细胞 细胞培养 细胞移植 

分 类 号：R687.4[医药卫生—骨科学] R622.9[医药卫生—外科学]