Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)  

作　　者：张之文 孙修勤 张进兴 昝金东 

机构地区：[1]College of Marine Life Sciences, Ocean University of China,Qingdao 266003, China [2]The First Institute of Oceanography, State Oceanic Administration of China,Qingdao 266061, China

出　　处：《Chinese Journal of Oceanology and Limnology》2008年第1期50-53,共4页中国海洋湖沼学报（英文版）

基　　金：Supported by the Project under Major State Basic Research of China (No. G199901205)

摘　　要：The prion protein (PrP) encoding gene of flounder (Paralichthys olivaceus) was cloned. It was not interrupted by an intron. This gene has two promoters in its 5' upstream, indicating that its transcription may be intensive, and should have an important function. It was expressed in all 14 tissues tested, demonstrating that it is a house-keeping gene. Its expression in digestion and reproduction systems implies that the possible prions of fish may transfer horizontally.The prion protein （PrP） encoding gene of flounder （Paralichthys olivaceus） was cloned. It was not interrupted by an intron. This gene has two promoters in its 5＇ upstream, indicating that its transcription may be intensive, and should have an important function. It was expressed in all 14 tissues tested, demonstrating that it is a house-keeping gene. Its expression in digestion and reproduction systems implies that the possible prions of fish may transfer horizontally.

关 键 词：Bastard halibut Paralichthys olivaceus prion protein PRP GENE CLONE expression 

分 类 号：S917.4[农业科学—水产科学]