环孢素A对吻合血管异体骨移植血管平滑肌细胞表型转变的影响  

Phenotypic modulation in the donor vascular smooth muscle cells after vascularized bone allograft

作　　者：陶圣祥[1] 陈振光[1] 谢昀[1] 郑晓晖[1] 杨玉华[1] 潘峰[1] 

机构地区：[1]武汉大学中南医院显微骨科 武汉大学医学部显微外科研究所,430071

出　　处：《中华创伤骨科杂志》2008年第2期150-153,共4页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金资助项目（30170946）

摘　　要：目的通过研究环孢素A对吻合血管异体骨移植血管平滑肌细胞（VSMCs）表型改变的影响，探讨移植物血管动态变化的规律。方法建立兔吻合血管异体骨移植修复股骨大段缺损的模型，动物分为实验组（术后应用环孢素A）及对照组（术后未应用环孢素A），在不同时段取出移植物血管制作组织学切片，观察血管壁形态和结构变化，研究VSMCs的表型改变。结果实验组和对照组的供体血管都呈进行性狭窄，血管内膜逐渐增厚，但实验组血管壁增厚的速度明显慢于对照组。实验组血管平滑肌细胞收缩型标志蛋白仅一肌动蛋白呈递减性表达；对照组也表现出同样的规律，但衰减速度明显快于实验组。结论吻合血管异体骨移植后移植物血管发生进行性狭窄；环孢素A能在一定程度上抑制VSMCs表型的转变，延长移植物通血时间。Objective To study the phenotypic modulation in the donor vascular smooth muscle cells （VSMCs） after vascularized bone allograft. Methods Animal models were used to conduct vascularized bone allograft to repair massive defects at the rabbit femoral shaft. Animals were divided into the experimental group, in which Cyclosporine A was applied after operation, and the control group, in which Cyclosporine A was not applied. Vascular samples taken from the donors were examined histologically to observe the changes in the vessel of the donor, and the phenotypic modulation in the VSMCs at different stages. Results The gradual development of vascular stenosis and thickening of inner membrane were observed in both the experimental group and the control group. But the thickening in the experimental group was significantly slower than that in the control group. The α-actin in the VSMCs was expressed in a decreasing manner in both groups, but the decreasing speed in the control group was obviously higher than in the experimental group. Conclusions Gradual development of vascular stenosis occurs in the donor＇s vessels after vascularized bone allograft. Cyclosporine A can inhibit the phenotypic modulation in the donor VSMCs, thus prolonging the time of blood circulation.

关 键 词：骨移植 环孢素A 骨缺损 

分 类 号：R687.3[医药卫生—骨科学]