Polypeptide Daintain as a New Biomarker for Detecting Breast Tumor  

作　　者：LI Fafang XIA Heshun CHEN Zhengwang 

机构地区：[1]Institute of Biophysics and Biochemistry, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China [2]Experimental Center, Youjiang Medical College for Nationalities, Baise 533000, Guangxi, China [3]Department of Pathology, Hubei Cancer Hospital, Wuhan 430079, Hubei, China

出　　处：《Wuhan University Journal of Natural Sciences》2008年第1期118-122,共5页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Natural Science Foundation of China (30370647, 30470823)

摘　　要：Daintain, a novel bioactive peptide produced and secreted by macrophages, was expressed in breast tumor tissues. The spatial distributions of daintain in 66 breast tumor specimens were investigated with immuno-histochemistry method. Reverse transcription polymerase chain reaction （RT-PCR） and in situ hybridization inspection system were also used to detect daintain in 45 cases of malignant breast tumors. The final results show that 93% high positive responses to daintain on breast cancer tumors. RT-PCR demonstrated that, no smear of daintain transcripted in benign tissues was found, and light smear in peri-cancer tissue was observed. Distribution of daintain was distinguishable among benign tissues, hyperplasia tissues, immature hyperplasia and invasive breast cancer, which can be used to mark the progression of the malignant lesion development. We conclude that the expression of daintain is up-regulated in breast cancers, which indicates that the peptide is closely associated with the disease progression. So daintain could be used as the biomarker for detecting breast cancer.Daintain, a novel bioactive peptide produced and secreted by macrophages, was expressed in breast tumor tissues. The spatial distributions of daintain in 66 breast tumor specimens were investigated with immuno-histochemistry method. Reverse transcription polymerase chain reaction （RT-PCR） and in situ hybridization inspection system were also used to detect daintain in 45 cases of malignant breast tumors. The final results show that 93% high positive responses to daintain on breast cancer tumors. RT-PCR demonstrated that, no smear of daintain transcripted in benign tissues was found, and light smear in peri-cancer tissue was observed. Distribution of daintain was distinguishable among benign tissues, hyperplasia tissues, immature hyperplasia and invasive breast cancer, which can be used to mark the progression of the malignant lesion development. We conclude that the expression of daintain is up-regulated in breast cancers, which indicates that the peptide is closely associated with the disease progression. So daintain could be used as the biomarker for detecting breast cancer.

关 键 词：daintain breast cancer IMMUNO-HISTOCHEMISTRY reverse transcription polymerase chain reaction（RT-PCR） in situ hybridization 

分 类 号：Q5[生物学—生物化学]