亮-脑啡肽抗体在不同脑区对大鼠氧惊厥的影响(论著)  

Effects of injection of leu-enkephalin antibody into different brain areas on oxygen convulsions in rats

作　　者：练庆林[1] 胡长虹[1] 倪国坛 陶恒沂 

机构地区：[1]第二军医大学海医系潜水生理教研室,上海市200433

出　　处：《中华航海医学杂志》1997年第2期83-86,共4页

基　　金：国家自然科学基金!38970319

摘　　要：目的：探讨亮－脑啡肽（L－Enk）抗体在纹状体、下丘脑、中脑和海马对大鼠氧惊厥的影响。方法：120只大鼠被随机分为12组，先在纹状体、下丘脑、中脑和海马分别注射微量L－Enk抗体或生理盐水，然后将动物暴露在0．6MPa（6ATA）高压氧（HBO）下，观察各组大鼠发生氧惊厥的潜伏期、惊厥发作的程度及动物的存活时间。结果：在纹状体和下丘脑，与对照组相比能够显著延长大鼠发生氧惊厥的潜伏期，减轻惊厥发作的程度和延长动物存活的时间（P＜0．05～0．01）；而中脑和海马与对照组比则相差不显著（P＞0．05）。结论：在大鼠纹状体和下丘脑分别注入微量L－Enk抗体，对氧惊厥具有一定的预防作用，提示大鼠氧惊厥的发生与纹状体和下丘脑内的L－Enk含量相关。Aim: To investigate the effects of injection of Leu-Enkephalin (L-Enk ) antibody into striatum, hypothalamus, mesencephalon and hippocampus on oxygen convulsions in rats. Methods: One hundred and twenty male SD rats were randomly divided into 12 groups. L-Enk antibody(0. 5 μl or 1. 0μl)or saline were dricro-injected into striatum, hypothalamus, mesencephalon and hippocampus respectively in different groups before the animals were exposed to 0. 6 MPa (6 ATA) hyperbaric oxygen (HBO). The latent period of convulsions (LPC),the severity of convulsions (SC) and the survival time (ST) of animals in each group were observed' Results:As compared with respective control groups, the LPC was significantly prolonged,the SC was significantly mitigated and the ST was significantly increased in the striatum and hypothalamus experiment groups (Pr0. 05~0.01 ),while there were no significant difference in the mesencephalon and hippocampus experiment groups (P> 0. 05 ). Conclusion:There were considerable oxygen convulsions preventive effect with the micro-injection of L-Enk antibody into striatum and hypothalamus. The results suggested that the oxygen convulsions of rats were related to the contents of L-Enk in striatum and hypothalamus.

关 键 词：氧惊厥 氧中毒 脑啡肽 抗体 

分 类 号：R845.230.2[医药卫生—航空、航天与航海医学]