鸭病原性大肠杆菌Ⅰ型及P型菌毛表达条件的研究  

Study on expression conditions of TypeⅠand Type P fimbria of pathogenic Escherichia coli isolated from ducks

作　　者：于学辉[1] 张焕容[1] 汤承[1] 杨晓农[1] 匡学谦[1] 杨治国[1] 程安春[2] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川农业大学动物医学院禽病防治研究中心

出　　处：《西南民族大学学报（自然科学版）》2008年第1期88-93,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：国家科技攻关重大项目(2004BA901A03);四川省重点建设学科项目(SZD0418);四川省畜牧食品局重点攻关课题"集约化养鸭场重大疫病防制关键技术的研究"

摘　　要：为探讨鸭病原性大肠杆菌Ⅰ型及P菌毛在体外的表达条件,用三株分别带有Ⅰ型菌毛编码基因(fim+),Ⅰ型和P型菌毛编码基因(fim+/papA+)和P型菌毛编码基因(papA+)的鸭病原性大肠杆菌SWY-098、SWY-009、SWY-027菌株,通过对鸡、鸭和豚鼠RBC的MSHA和MRHA试验,分别从培养温度、培养时间和培养基三个方面对菌毛在体外的表达进行优化.结果表明:三株鸭病原性大肠杆菌在37℃表达菌毛,在18℃不表达菌毛;用BBL培养基37℃静止培养48 h是I型和P型菌毛在体外表达最适宜条件.To evaluate the expression conditions of Type Ⅰ and Type P fimbria of pathogenic Escherichia coli （E. coli） isolated from ducks, three strains of pathogenic E. coli SWY-098, SWY-009 and SWY-027 derived from ducks are used to conduct the MSHA and MRHA experiments of RBC from chicken, duck and guinea pig, and the strains mentioned above possess coding genes of Type Ⅰ fimbria （fim^＋）, Type Ⅰ and Type P fimbria （fim^＋/papA ^＋）, and Type P fimbria （papa ^＋） respectively. The expression conditions of fimbria in vitro, such as culture temperature, culture time and medium are optimized. The results show that the three strains of E. coli can express their fimbria at the temperature of 37℃ and without expression at 18℃, and the stationary culture in BBL medium at 37℃ for 48h is the optimum expression condition of Type Ⅰ and Type P fimbria in vitro.

关 键 词：鸭 病原性大肠杆菌 菌毛 表达条件 

分 类 号：S858.32[农业科学—临床兽医学]