新城疫病毒F基因重组鸡痘病毒的安全性及免疫效力  

Safety and immune efficacy of recombinant fowlpox virus expressing fusion protein of Newcastle disease virus

作　　者：张体银[1] 王光锋[1] 孙蕾[1] 梁萍[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室

出　　处：《中国兽医科学》2008年第2期116-119,共4页Chinese Veterinary Science

基　　金：国家高技术研究发展计划(863)项目(2001AA213041);科技部农业科技成果转化项目(OIEFN213201078)

摘　　要：将重组鸡痘病毒rFPVLP-SF分别皮下接种1日龄SPF鸡和26日龄商品鸡,评价其安全性和免疫效力。SPF鸡接种后除282E4株鸡痘病毒组在接种部位出现痘斑外,其他组鸡无任何临床症状。抗体检测结果表明,免疫接种后rFPVLP-SF组鸡的血清抗体水平没有显著变化,免疫后第21 d以1×105ELD50的F48E8株NDV滴鼻攻毒,观察14 d,rFPVLP-SF和油乳剂灭活疫苗免疫鸡的保护率分别为92%和100%,二者没有显著差异;大空斑株鸡痘病毒组和攻毒对照组鸡均全部死亡。结果显示,该重组病毒具有良好的安全性和免疫效力,有一定的应用前景。One-day-old SPF chickens（4 groups of 8 each） or 26-day-old commercial layers（4 groups of 25 each） were immunized,respectively with the recombinant fowlpox virus（rFPV,.e-SF） expressing fusion protein of ZJ-1 strain of Newcastle disease virus（NDV） by subcutaneous route at the dosage of 1 × 10^5PFU in order to evaluate, respectively,its safety and immune efficacy. With the exception of the SPF chickens in- oculated with FPV strain 282E4, the SPF chickens had no any clinical symptoms. Detection of a antibody indicated that serum antibodies level to rFPVLP-SF of NDV slightly changed after inoculation in the chickens and all commercial chickens were challenged intranasally with 1 × 10^5 ELD50 of F48E8 strain NDV on day 21 after vaccination. The protection rates of rFPVLP-SF and inactivated vaccine in oil emulsion were 92% and 100%,respectively,without significant difference（P〉0.05）, while all of the chickens vaccinated with Large plaque strain of Fowlpox virus（FPVLp） and unvaccinated died. The results showed that rFPVLP- SF was safe and effective for protecting chickens from ND.

关 键 词：新城疫病毒 F基因 重组鸡痘病毒 安全性 免疫效力 

分 类 号：S852.659.5[农业科学—基础兽医学]