地表水细菌总数检测方法探讨  

Evaluation of Methods for Testing Sum of Bacteria in Surface Water

作　　者：徐飞 叶友红[2] 江迎春[3] 刘洋5 

机构地区：[1]绍兴市水务集团技术质量中心,浙江绍兴312000 [2]浙江浙大中控信息技术有限公司,浙江杭州310000 [3]浙江工业大学生命环境学院

出　　处：《净水技术》2007年第6期66-69,共4页Water Purification Technology

摘　　要：采用3种不同接种方法检测地表水细菌,对比检测结果表明:传统的倾注法检出率低;涂布法菌落分布不均,影响计数;采用新的接种方法—双层培养法不仅检出率高,而且菌落分散,有利于计数。3种方法之间存在显著性差异。此外,增加培养基营养成分和稀释时增加检样量均能提高细菌检出数量。The bacteria counting results were studied and compared between different inoculation methods： the smearing method ,the plate pouring method and the double layer method. The double layer method was characterized by higher accuracy and sensitivity than the other two methods. Optimizing composition of culture media and taking larger volume of water sample can also raise the sensitivity and the counting number.

关 键 词：检样量 倾注法 涂布法 双层培养法 t检验法 

分 类 号：X832[环境科学与工程—环境工程] S858.31[农业科学—临床兽医学]