银杏叶提取物对PGF2α诱导心肌细胞肥大作用的研究  

Effect of Extract of Ginkgo Biloba(EGb)on Cell of Neonatal Rat Cardiocyte Hypertrophy Induced by Prostaglandin F2 Alpha

作　　者：王海霞[1] 代文静[1] 程翔[1] 冯义柏[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科

出　　处：《医学研究杂志》2008年第2期58-60,共3页Journal of Medical Research

基　　金：国家自然科学基金资助课题(30600234)

摘　　要：目的研究银杏叶提取物EGb(Extract of Ginkgo Biloba,EGb)对前列腺素F2α(PGF2α)所致心肌细胞肥大的作用及作用机制。方法实验选用正常培养的乳鼠心肌细胞作为对照组,PGF2α和PGF2α加不同浓度EGb作为实验组,在培养的新生乳鼠心肌细胞上,采用Bradford蛋白浓度测定试剂盒检测心肌细胞总蛋白含量;相差显微镜检测细胞面积;荧光倒置显微镜测定心肌细胞内活性氧(reactive oxygen species.ROS)的活性。结果PGF2α10-7mol/L使心肌细胞面积明显增大和蛋白质含量明显增加(P<0.05),并使细胞内活性氧显著升高。与PGF2α组比较,银杏叶提取物EGb(40μg/ml,80μg/ml,100μg/ml)组可分别使心肌细胞面积缩小19%,27%,33%(P<0.05);蛋白质含量下降6%,19%,24%(P<0.05);心肌细胞内活性氧荧光密度的活性下降21%,39%,47%(P<0.01)。结论银杏叶提取物EGb可抑制PGF2α诱导的心肌细胞肥大,该作用可能与其抑制细胞内氧自由基活性有关。Objective To investigate the effects and mechanisms of extract of ginkgo biloba（EGb） on the cell of cardiocyte hypertrophy induced by prostaglandin F2 alpha. Methods normal cells were used as negative control, and spontaneously PGF2α and EGb were used as experimental groups. The cells were isolated and cultured on cultured neonatal rat cardiocyte by PGF2α, cardiocyte hypertrophy was determined by the cell surface and the total protein of cells ; the level of intracellular ROS measured by the fluorescence microscope. Results In cardiocyte hypertrophy, the cell surface , the level of intracellular ROS and the total protein of cells increased significantly in cutured neonatal rat cardiac treated with PGF2α. Compared with cells treated with PGF2α, EGb（40μg/ml, 80μg/ml, 100μg/ml） inhibited cardiocyte hypertrophy by PGF2α -induced, decreased in the cell surface by 19% ,27% ,33% and in the total protein of cells by 21% ,39% ,47% respectively （all P 〈 0. 01 ） , and in the fluoreacence intensity of intracellular DCF - DA by 21% ,39% ,47% （P〈0.01 ）. Conclusions EGb could suppress cardiocyte hypertrophy induced by PGF2α. The suppression of intracellular ROS activity could be concerned with cardiocyte hypertrophy induced by PGF2α.

关 键 词：银杏叶提取物 前列腺素F2Α 心肌肥大 活性氧 

分 类 号：R542.2[医药卫生—心血管疾病] R285.5[医药卫生—内科学]