3种小麦纹枯病病原菌接种方法的比较  

Comparative Effects of Different Inoculation Methods on Sharp Eyespot Fungus (Rhizoctonia cerealis) in Wheat

作　　者：颜伟[1] 蔡士宾[1] 吴纪中[1] 张先义[1] 吴小有[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏南京210014

出　　处：《长江大学学报（自科版）（中旬）》2007年第4期1-4,共4页Journal of Yangtze University(Nature Science Edition)

基　　金：国家"十五"攻关项目(2004BA525B03);江苏省自然科学基金资助项目(BK2004170)

摘　　要：为使小麦纹枯病鉴定方法简单、科学、可靠,选用3种接种纹枯病菌的方法,即沟带接种法、土表接种法、牙签嵌入接种法,比较了不同抗性水平品种、不同接种方法之间发病率、病情指数的差异。结果表明:不同接种方法之间、不同品种之间发病率、病情指数存在显著差异。沟带接种法简单、粗放,受环境的影响较大,可以用于大量材料的初选。牙签嵌入接种法的发病率最高,平均发病率在90%以上,重复之间差异不显著,接种容易成功,重复性好,但试验鉴定要求细致、繁琐,工作量大。土表接菌法最为简单、方便,通过适当控制试验条件,可以获得满意的接种效果。在实际研究过程中,可根据自身条件和试验要求选择合适的接种方式。In order to identify the comparative effects of different inoculation methods on sharp eyespot fungus (Rhizoctonia cerealis) in wheat,three inoculation methods,i.e. sowing-pitch method (applying inoculums in sowing-pitch when seeding),toothpick method (inserting affected toothpicks between sheath and stem at booting),soil surface method (applying inoculums on soil surface in early spring),were performed in different wheat genotypes. The results showed that there were significant difference among three inoculation methods and wheat genotypes. Sowing-pitch method could be used in preliminary large-scale screening as it is easy to hand. Toothpick method could induce the highest disease incidents with highly repeatable results. It could be used in genetic studies and small-scale evaluation. However,more workload were required in this method. Soil surface method combined the advantages of toothpick method and sowing-pitch method and was the handiest and most effective method to evaluate wheat sharp eyespot.There were significant differences in disease incident among genotypes. SW038,WHO6-7,Niavt14 and CI12633 showed high resistance under three methods and could be used as resistant parents in wheat breeding.

关 键 词：小麦 纹枯病 接种方法 

分 类 号：S435.121.49[农业科学—农业昆虫与害虫防治]