L-精氨酸对兔肺缺血再灌注时血红素氧合酶-1的影响  

Effect of L-arginine on heme oxygenase-1 in the lung after pulmonary ischemia-reperfusion in rabbits

作　　者：王万铁[1] 邱晓晓 戴雍月[1] 郝卯林[1] 王青[1] 

机构地区：[1]温州医学院病理生理学教研室,325035

出　　处：《中华麻醉学杂志》2008年第1期75-77,共3页Chinese Journal of Anesthesiology

基　　金：浙江省跨世纪学术和技术带头人培养基金资助项目（992086）;温州市“551人才工程”培养基金资助项目（98113）;浙江省教育厅科研基金资助项目（20000670）;温州市科技计划重点项目（Y2005A080）

摘　　要：目的探讨L1广精氨酸对兔肺缺血再灌注时血红素氧合酶-1（HO-1）的影响。方法健康日本大耳白兔20只，雌雄不拘，体重1．7～2．6kg，随机分为2组，肺缺血再灌注组（IR组）和L-广精氨酸治疗组（L-Arg组），每组10只。建立兔单肺原位缺血再灌注损伤模型，L-Arg组肺缺血前20min耳缘静脉注射LArg溶液100mg／kg。采用原位杂交方法测定兔肺组织HO-1的表达；计算肺组织湿／干重比（W／D）及损伤肺泡百分数（IAR）；电镜下观察肺组织超微结构。结果HO-1在两组肺血管内皮、部分血管平滑肌、外膜层及部分气道上皮均有表达；与IR组比较，L-Arg组HO-1水平升高，W／D及IAR降低（P〈0．05）；L-Arg组肺组织形态学异常改变轻于IR组。结论L广精氨酸可通过上调肺组织HO-1的表达，增强肺组织HO-1的活性，减轻兔肺缺血再灌注损伤。Objective To investigate the effect of L-arginine （L-Arg） on the expression and activity of heine oxygenase-1 （HO-1） in the lung tissue after pulmonary ischemia-reperfusion （I/R）. Methods Twenty healthy Japanese long-ear white rabbits of both sexes weighing 1.7-2.6 kg were randomly divided into 2 groups （n = 10 each）： group Ⅰ pulmonary I/R and groupⅡ L-Arg ＋ I/R. The animals were anesthetized, tracheostomized and mechanically ventilated. Lung ischemia was induced by ligation of the blood vessels and bronchus in the hilum of the left lung for 1 h followed by 3 h reperfusion according to Sekido. In L-Arg group the animals received L-Arg 100 mg/kg 20 rain before ischemia. The animals were killed at the end of 3 h reperfusion and the lungs were removed for determination of HO-1 expression in the lung, W/D lung weight ratio and the injured alveoli rate （IAR） and microscopic examination with light and electron microscope. Results HO-1 expression was detected in the pulmonary endothelial cells, pulmonary vascular smooth muscle, extima of vessels and epithelial cells of airway in both groups. The absorbance and activity of HO-1 were significantly higher while W/D ratio and IAR values were significantly lower in L-Arg group than in I/R group. The morphological abnormality was less severe in L-Arg group than in I/R group.Conclusion L-Arg can protect the lung against I/R injury by up-regulating HO-1 expression and increasing HO-1 activity in the lung.

关 键 词：精氨酸 肺 再灌注损伤 血红素氧化酶(脱环) 

分 类 号：R686[医药卫生—骨科学]