金黄色葡萄球菌毒素基因的检测及临床应用  

Detection of Staphylococcus aureus toxigenes and its clinical application

作　　者：蔡朝阳[1] 马筱玲[2] 纪冰[2] 赵燕[3] 

机构地区：[1]蚌埠医学院微生物学教研室,安徽蚌埠233030 [2]安徽省立医院检验科,安徽合肥230001 [3]安徽医科大学第一附属医院检验科,安徽合肥230022

出　　处：《蚌埠医学院学报》2008年第1期9-12,共4页Journal of Bengbu Medical College

基　　金：安徽省科技攻关项目(07010302192)

摘　　要：目的:了解医院获得性金黄色葡萄球菌(HA-SA)和社区获得性金黄色葡萄球菌(CA-SA)所携带肠毒素、表皮剥脱性毒素(ETs)、中毒性休克综合征毒素-1(TSST-1)及杀白细胞素基因(PVL)的特点。方法:应用PCR法检测肠毒素A、B、C、D、E基因,ETs A、B基因,TSST-1以及PVL。结果:116株金黄色葡萄球菌中,85株为HA-SA,检出肠毒素A基因6株,肠毒素C基因1株,肠毒素D基因1株,PVL2株;31株为社区获得性金黄色葡萄球菌,检出肠毒素A基因1株,肠毒素B基因1株,肠毒素D基因1株,PVL7株;所有菌株均未检测到肠毒素E、ETs A、B和TSST-1基因。CA-SA PVL检出率显著高于HA-SA,两者比较差异有统计学意义(P<0.005),而肠毒素、ETs和TSST-1基因检出率比较差异均无统计学意义(P>0.05)。结论:金黄色葡萄球菌可分泌多种毒素,在分离金黄色葡萄球菌的同时,应注意其毒素检测。Objective：To study genic feature of Staphylococcal enterotoxins （SEs）, exfoliative toxins （ETs）, toxic shock syndrome toxin 1 （ TSST-1 ） and Panton-Valentine leukociclin（PVL） carried by both hospital-acquired Staphylococcus aureus（HA-SA） and communityacquired Staphylococcus aureus （CA-SA）. Methods： The genes of SEA-SEE, ETA, ETB, TSST-1 and PVL were detected by PCR. Results：Out of 116 SA strains,85 strains were HA-SA. SEA was detected in 6 strains,SEC in 1 strain,SED in 1 strain,and PVL in 2 strains. Thirty one strains were CA-SA. SEA was detected in 1 strain, SEB in 1 strain, SED in 1 strain, and PVL in 7 strains. SEE, ETA, ETB and TSST-1 were negative in all SA. PVL positive rate detected in CA-SA was much higher than that in HA-SA （ P 〈 0. 005 ）, while positive rates of SEs, ETs ,TSST-1 were not significant different between CA-SA and HA-SA （P 〉 0. 05 ）. Conclusions：Staphylococcus aureus may secrete various toxins ,detecting should pay attention to whose toxins while separating Staphylococcus aureus.

关 键 词：金黄色葡萄球菌 肠毒素 表皮剥脱性毒素 中毒性休克综合征毒素-1 杀白细胞素 基因 

分 类 号：R378.11[医药卫生—病原生物学]