辽细辛提取物对拟盘多毛孢菌的离体抑制作用  

The Antibacterial Effects of Asarum heterotropoides Extracts to Pestaloliopsis sp. in vitro

作　　者：王桂清[1] 张军华[2] 张敏[2] 姬兰柱[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]聊城大学农学院,山东聊城252059

出　　处：《河南农业科学》2008年第2期60-63,114,共5页Journal of Henan Agricultural Sciences

基　　金：中国科学院知识创新方向性项目(KZCX1-SW-19);中国科学院农办重点项目

摘　　要：采用生长速率法和孢子萌发法测定了辽细辛根的不同溶剂(石油醚、氯仿、乙酸乙酯、乙醇)粗提取物和精油对引起牡丹叶斑病的拟盘多毛孢菌菌丝生长和孢子萌发的作用。结果表明,细辛不同溶剂提取物和精油对拟盘多毛孢菌的菌丝生长和孢子萌发均有一定的抑制作用,且对孢子萌发的抑制效果好于对其菌丝生长的抑制效果;石油醚提取物和精油的抑菌效果较好,对菌丝生长的EC50分别为94.91 mg/L和120.44 mg/L,对孢子萌发的EC50分别为132.64 mg/L和110.66 mg/L。说明细辛所含的主要抑菌活性成分易被极性小的有机溶剂所提取,细辛精油中也含有抑菌活性物质。The effects of asarum essential oils and extracts derived from 4 different solvents （petroleum ether, chloroform, ethyl acetate and ethanol） on the hyphae growth and spore germination of Pestaloliopsis sp. were studied using the growth rate method and spore germination method. The results showed that the asarum extracts and essential oils have significant antibacterial activi- ty to tested pathogens, with a higher effect to spore germination than to hyphae growth. The antibacterial effects of petroleum ether extract and essential oils were the best： EC50 =94.91 mg/L and EC50=120.44 mg/L for hyphae growth,and EC50= 132.64 mg/L and EC50 = 110.66 mg/L for spore germination, respectively. These suggested that the antibacterial activity substances in herba asari root are easily extracted by the solvents with lower polarity （i. e. petroleum ether） and the asarum essential oils also contain the antibacterial activity substances.

关 键 词：辽细辛 拟盘多毛孢菌 生长速率法 孢子萌发法 

分 类 号：Q949.742.3[生物学—植物学]