藏绵羊GHRH基因部分片段的克隆及HaeⅢ-RFLP分析  

Cloning and HaeⅢ-RFLP Analysis of Partial GHRH Gene in Oura-Type Tibetan Sheep

作　　者：马志杰[1] 魏雅萍[1] 钟金城[2] 杨万远[2] 常怀普[3] 陈生梅[1] 

机构地区：[1]青海省畜牧兽医科学院畜牧研究所,青海西宁810016 [2]西南民族大学生命科学与技术学院,四川成都610041 [3]西南大学生物技术学院,重庆400715

出　　处：《河南农业科学》2008年第2期95-98,共4页Journal of Henan Agricultural Sciences

基　　金：青海省科技厅基础研究与软科学计划项目资助(2006-Z-601)

摘　　要：对欧拉型藏绵羊生长激素释放激素(GHRH)基因部分片段进行PCR扩增、纯化和克隆测序,利用GenBank中的Blastn方法与普通牛相应基因序列进行了比对分析,并对54只欧拉型藏绵羊该基因片段进行了HaeⅢ-RFLP研究。结果表明:欧拉型藏绵羊与普通牛GHRH基因部分序列间同源性为93%;序列间碱基变异类型主要表现为碱基转换和颠换,亦存在碱基插入和缺失;54只欧拉型藏绵羊该基因片段HaeⅢ-RFLP分析均为单态,表现为AA基因型。The partial sequence of Oura-type Tibetan sheep GHRH gene was sequenced （GenBank Accession No： EU156338）and compared with that of cattle （Bos taurus ） through blastn method. The results showed that the nucleotide homology of the partial intron Ⅰ of GHRH gene between the Tibetan sheep and the cattle was up to 93% ,and the main mutation types were base transition and transversion though there also existed some base invertions and deletions. Meanwhile, the polymorphism of partial intron Ⅰ of GHRH gene in 54 Oura-type Tibetan sheep （Ovis aries） was analyzed by HaeⅢ-RFLP method,which indicated that no polymorphism was detected in this fragment and all genotypes of Oura-type Tibetan sheep studied were AA genotype.

关 键 词：欧拉型藏绵羊 生长激素释放激素 克隆 PCR-RFLP 

分 类 号：S826[农业科学—畜牧学]