药用百合鳞叶中总DNA提取方法的研究  

Extraction of Total DNA from Medicinal Lilii's Squama Leaves

作　　者：童巧珍[1] 周日宝[2] 刘湘丹[2] 盛孝邦[1] 高彦宁[2] 

机构地区：[1]湖南农业大学,长沙市410028 [2]湖南中医药大学,长沙市410007

出　　处：《中国药房》2008年第6期406-408,共3页China Pharmacy

基　　金：国家科技攻关项目资助课题(2006BAI06A11-06);湖南省教育厅资助项目(06C626)

摘　　要：目的：对药用百合DNA的提取过程进行改良和优化，筛选出较理想的DNA提取方法。方法：在传统十六烷基三甲基氯化铵（CTAB）提取方法基础上，进行改良和优化，寻找出一种实用的DNA提取方法。结果：用改良CTAB法提取的总DNA的OD260／OD280在1．6～2．0之间，电泳条带清晰，其含量和纯度完全满足引物扩增分析的要求。结论：本试验中的百合鳞叶总DNA的提取方法简便实用，所得DNA的产量和纯度均能满足基因工程操作的要求。OBJECTIVE： To improve and optimize the extraction process of DNA from medicinal lily. METHODS： A new feasible DNA extraction method was available by modifying and optimizing the traditional CTAB extraction method. RESULTS： The OD260/OD280 of the total DNA extracted by the modified CTAB method ranged from 1.6 to 2.0 with clear electrophoresis strip, and its content and purity were all up to the standards for RAPD analysis.CONCLUSION： A simple and feasible extraction process of DNA from medicinal Lily was found out and the content and purity of the DNA extracted in this method can meet the requirements for gene engineering.

关 键 词：药用百合 鳞叶 DNA提取 

分 类 号：R31[医药卫生—基础医学]