正交试验优选定心藤中总黄酮提取与纯化工艺  

Optimization of the Extraction and Purification Techniques of Total Flavonoids in Mappianthus Iodoies by Orthogonal Experiment

作　　者：曾立[1] 尹文清[1] 王安伟[1] 周中流[1] 

机构地区：[1]广西师范大学化学化工学院,桂林市541004

出　　处：《中国药房》2008年第6期418-420,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(30460153)

摘　　要：目的:优选定心藤中总黄酮提取和纯化工艺。方法:通过紫外分光光度法测定总黄酮含量,并采用正交设计试验法确定溶剂浓度、固液比、提取时间、提取次数对定心藤总黄酮提取的影响大小。同时,黄酮粗品用D-101大孔树脂纯化。结果:最佳提取工艺条件为乙醇浓度65%,固液比1∶10,提取2.5h,重复提取3次。黄酮粗品纯化后纯度可以达到86%以上。结论:本试验可为定心藤进一步研究和资源开发利用提供一定的科学依据。OBJECTIVE： To optimize the extraction and purification techniques of total Flavonoids in Mappianthus iodoies .METHODS： The content of total Flavonoids was determined by UV spectrophotometry. Orthogonal experiment was carried out to investigate the effects of concentration of solvent, the ratio material to liquid, time length of extraction, and frequency of extraction on extraction results of total Flavonoids from Mappianthus iodoies . Under this condition, by means of D- 101 macroporous resin, the crude Flavonoids extraction was purified. RESULTS： The best extraction conditions were as follows： using 65% （V/V） ethanol as extractor with the ratio of material to liquid at 1：10,and extracting for 2.5 h/time for 3 times. The content of total Flavonoids in the refined product was above 86%. CONCLUSION： This study provided scientific basis for further research, development and utilization of Mappianthus iodoies.

关 键 词：定心藤 总黄酮 提取 纯化 

分 类 号：R284.2[医药卫生—中药学]