RP-HPLC法同时测定不同品种淫羊藿药材中5种主要黄酮类成分的含量  

Simultaneous Determination of Five Main Flavonoids in Herba Epimedii from Different Species by RP-HPLC

作　　者：陈彦[1] 赵艳红[2] 贾晓斌[2] 丁安伟[1] 

机构地区：[1]南京中医药大学,南京市210046 [2]江苏省中医药研究院/江苏省现代中药制剂工程技术研究中心,南京市210028

出　　处：《中国药房》2008年第6期431-433,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(30572372)

摘　　要：目的:建立以反相高效液相色谱法测定不同品种淫羊藿药材中5种主要黄酮类成分含量的方法。方法:色谱柱为Zorbax SB-C18(250mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),检测波长为270nm,柱温为30℃,同时测定淫羊藿药材中淫羊藿苷、朝藿定A、朝藿定B、朝藿定C、宝藿苷Ⅰ的含量。结果:5种成分均能达到基线分离,线性良好。不同品种淫羊藿药材中5种主要黄酮类成分的含量差别较大。结论:建议在考察淫羊藿药材的质量时,不能只考察淫羊藿苷,应建立多种黄酮类成分测定指标,以全面控制淫羊藿药材的质量。OBJECTIVE： To determine five main Flavonoids （Icariin, Epimedin A, Epimedin B, Epimedin C, Baohuoside Ⅰ） simultaneously in Herba Epimedii from different species by RP- HPLC. METHODS： The samples were separated on Zor- bax SB-C18（250 mm × 4.6 mm, 5μm） with mobile phase consisted of acetonitrile-water （gradient elution） . The detection wavelength was 270 nm and the column temperature was 30 ℃ .The contents of Icariin, Epimedin A, Epimedin B, Epimedin C, Baohuoside Ⅰ in Herba Epimedii were determined simultaneously. RESULTS： All the five Flavonoids reached baseline separation with good linearity. The contents of the five Flavonoids from different species varied significantly. CONCLUSION： The results suggest we should establish multiple indicators of Flavonoids instead of considering the index of Icariin only to achieve a full control on the quality of Herba Epimdii.

关 键 词：反相高效液相色谱法 淫羊藿 淫羊藿苷 朝藿定A 朝藿定B 朝藿定C 宝藿苷Ⅰ 含量 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]