抗风湿(炎)类中成药中添加化学成分的检验方法研究  

Detection of Chemical Constituents in Antirheumatic Traditional Chinese Medicines

作　　者：付萍萍[1] 刘惠军[1] 陈维壹 张月寒[1] 董秋香[1] 段春明[1] 

机构地区：[1]河北保定市药品检验所,保定市071051

出　　处：《中国药房》2008年第6期433-435,共3页China Pharmacy

基　　金：河北省科学技术研究与发展指导计划课题(06276437)

摘　　要：目的:建立抗风湿(炎)类中成药中同时检测醋酸泼尼松等7种化学成分的高效液相色谱法。方法:色谱柱为Hypersil BDS C18(200mm×4.6mm,5μm),流动相为甲醇-1g·L-1磷酸二氢钾溶液(用磷酸调pH(4.0±0.05))=58∶42,流速为1.0mL·min-1,检测波长为220nm。结果:7种成分均能得到较好的分离,且在一定的进样量范围内与各自峰面积积分值呈良好的线性关系。结论:本法快速、简便,能同时对7种化学成分进行定性、定量分析,可用于鉴定抗风湿(炎)中成药中是否添加化学成分。OBJECTIVE： To establish an HPLC method for the detection of Prednisone acetate, Hydrocortisone, Dexamethasone acetate, Piroxicam, Diclofenac sodium, Indometacin and Ibuprofen, the 7 chemical constituents in antirheumatic traditional Chinese medicine. METHODS： The chromatographic separation was performed on a Hypersil BDS C18 column （200 mm × 4.6 mm, 5 μm） using methanol - 1 g · L^ -1KH2PO4（58 ： 42） （adjusted to pH 4.0± 0.05 with H3PO4） as the mobile phase at a flow rate of 1.0 mL · min^-1. The detective wavelength was set at 220 nm. RESULTS： Seven chemical constituents were all well- separated with good linearity within respective range of sample size. CONCLUSION： The method is rapid and con- venient, and can be used for the quantitative and qualitative analyses of 7 chemical constituents in antirheumatic traditional Chinese medicine and to detect whether there is addition of chemical constituents in antirheumatic traditional Chinese medicines.

关 键 词：抗风湿(炎)类中成药 化学成分 高效液相色谱法 

分 类 号：R283[医药卫生—中药学] R927.1[医药卫生—中医学]