RP-HPLC法测定人血浆中辛伐他汀的浓度  

Determination of simvastatin in human plasma by RP-HPLC

作　　者：王健[1] 刘晓峰[1] 徐力生[2] 王惠成[1] 董玲[2] 

机构地区：[1]宁夏自治区人民医院,宁夏银川750021 [2]宁夏医学院,宁夏银川750021

出　　处：《宁夏医学杂志》2008年第2期158-159,共2页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(NZ07109)

摘　　要：目的建立人血浆中辛伐他汀的HPLC测定方法。方法采用Thermo-C18柱为分析柱;流动相为乙腈:水(60:40,V/V);流速为1.0 ml/min;检测波长为237 nm;柱温:35℃。结果辛伐他汀、洛伐他汀与人血浆中的内源性物质均实现较好分离,在0.5-25.0 ng/ml的范围内线性良好,r=0.9997(n=5),回收率为93.06%,日内及日间RSD均小于3.01%,最低检测限为0.25ng/ml。结论本法取样量小,操作简便快速,适用于临床辛伐他汀的血药浓度监测。Objective To establish a high - performance liquid chromatographic （HPLC） assay for the determination of simvastatin in human plasma. Methods Thermo - C18 column was used： the mobile phase consisted of acetonitrile -water （60：40, V/V）, the flow rate was 1.0 ml/min and the detection wavelength was at 237 nm at 35℃ temperature. Results A linearity was obtained from 0. 5 to 25.0 ng/ml of simvastatin in plasma with a good correlation coefficient （ r = 0. 9997, n = 5）. The mean recovery was 93.06%. The intra day and inter day RSDs were less than 3.01%, and detection limit was 0. 25 ng/ml. Conclusion The improved method is sensitive, specific and simple and the method is suitable for the pharmacokinetics monitor.

关 键 词：辛伐他汀 血药浓度 高效液相色谱法 

分 类 号：R96[医药卫生—药理学]