流式细胞术检测苦参素对人胃癌细胞系MGC-803凋亡的作用  

Detecting apoptosis on the human carcinoma cell line MGC -803 with flow cytometer（FCM）

作　　者：朱艳琴 徐玉芳 Zhu Yanqin, Xu Yufang. （ College of Basic Medical Sciences , Henan College of Traditional Chinese Medicine , Zhengzhou ,450008 ）

机构地区：河南中医学院基础医学院,河南郑州450008

出　　处：《中国保健》2008年第4期92-93,共2页Chinese Health Care

摘　　要：目的：运用流式细胞术检测苦参素对人胃癌细胞系MGC-803的凋亡作用。方法：运用流式细胞术检测细胞凋亡率及凋亡相关基因蛋白Bcl-2和Bax的表达。结果：苦参素1mg／ml、2mg／ml作用24h后，流式细胞仪检测结果显示，细胞凋亡率与阴性对照组相比。差异具有显著性（P〈0、05）；Bcl-2表达量明显减少，Bax表达量明显增加，差异具有显著性（P〈0．05）。结论：苦参素对人胃癌细胞系MGC-803有诱导凋亡作用，其机制与上调Bax和下调Bcl-2表达有关。Objective：To detecte apoptosis on the human carcinoma cell line MGC - 803 with flow cytometer. Methods：The rate of apoptosis and the expression level of Bel -2 and Bax by flow cytometry. Results： After MGC -803 cells were treated with lmg/m1,2mg/ml oxymatrine for 24h ,the expression of Bel -2 were reduced and the expression of Bax were increased remarkably （P 〈 0. 05 ）. Conclusion ：oxymatrine can induce of the human gastric eaneinoma cell line MGC - 803 by down - regulating expression of Bel - 2 and up - regulating expression of Bax.

关 键 词：流式细胞术 胃癌细胞 凋亡 Bcl-2 BAX 

分 类 号：R285.6[医药卫生—中药学;医药卫生—中医学]