葡萄糖转运蛋白1在乳腺癌组织中表达的临床意义  

Significant increase of glucose transport activity in breast cancer

作　　者：李娟[1] 杨守京[1] 赵玺龙[1] 张亚青[1] 李锴男[1] 崔继红[1] 李静[1] 

机构地区：[1]第四军医大学西京医院病理科基础部病理学教研室,西安710032

出　　处：《中华病理学杂志》2008年第2期103-108,共6页Chinese Journal of Pathology

摘　　要：目的探讨葡萄糖转运蛋白1（glucose transporter 1，Glut-1）及其mRNA在正常乳腺组织、乳腺纤维腺病、乳腺纤维腺瘤和乳腺癌中的表达及其意义。方法收集乳腺组织标本147例，其中乳腺浸润性导管癌92例、纤维腺瘤26例、腺病24例、正常乳腺组织5例，应用免疫组织化学EnVision法、Western blot以及RT-PCR分别定量检测不同病变组织中Glut-1蛋白及其mRNA表达水平。结果在正常乳腺组织、乳腺腺病以及纤维腺瘤组织中难以检测到Glut-1阳性表达或仅呈轻微胞质着色。在乳腺癌组织中，Glut-1表达强度明显增高（P=0．0002），主要定位于瘤细胞膜，细胞质染色较淡。原发灶及转移灶中央的细胞Glut-1表达明显高于周边癌组织，转移灶Glut-1表达水平明显高于原发灶，浸润癌高于原位癌。Western blot定量分析结果显示，乳腺癌组织中Glut-1表达水平显著高于纤维腺瘤（P=0．001）和腺病（P=0．001），各病变组织间差异有统计学意义（P=0．0002）。RT—PCR定量检测分析显示乳腺浸润性导管癌中Glut-1 mRNA的表达明显高于纤维腺瘤（P〈0．05）和腺病（P〈0．05），各病变组织间差异有统计学意义（P=0．0001）。结论乳腺癌组织中葡萄糖转运活性显著增高，Glut-1的表达水平与乳腺肿瘤的恶性程度、肿瘤细胞的浸润与转移密切相关；Glut-1高表达可作为细胞的恶性转化标志，并有望成为乳腺恶性肿瘤早期诊断、判断预后的一种新标记物及治疗的新靶点。Objective To study the expression level and significance of glucose transporter 1 （ Glut- 1 ） in normal breast tissue, adenosis, adenoma and breast carcinoma. Methods A total of 147 cases of female breast tissue samples, including 92 cases of invasive ductal carcinoma, 26 cases of breast fibroadenoma, 24 cases of breast adenosis and 5 cases of normal breast tissues, were collected for quantitative detection of the expression of Glut-1 protein by immunohistochemistry （EnVision method） and Western blot, and its mRNA by reverse transcriptase-polymerase chain reaction （RT-PCR）. Results In normal breast tissue and benign lesions of the breast, Glut-1 was undetectable or only weakly detectable in cytoplasm of ductal and acinar epithelia. In contrast, the intensity of Glut-1 staining was significantly higher in invasive ductal carcinomas （P =0. 0002） with protein expression predominantly in cellular membrane and lesser in cytoplasm. Western blot and RT-PCR analyses showed that the expression of Glut-1 protein and mRNA were significantly increased in invasive ductal carcinoma than fibroadenoma （P = 0. 001 for protein; P 〈 0.05 for mRNA） and adenosis （ P = 0. 001 for protein; P 〈 0. 05 for mRNA）. There was a significant difference among groups （P =0. 0002 for protein; P =0. 0001 for mRNA）. Conclusions Glucose transport activity, as indicated by Glut-1 protein and its mRNA expression, significantly increases in breast carcinoma than non-cancerous lesions. The over-expression of Glut-1 in breast carcinoma is tightly coupled with tumor cell proliferation, invasion and metastasis, implying that Glut-1 may serve as a new marker in the early diagnosis and prognostication of breast malignancy as well as a new therapeutic target.

关 键 词：葡萄糖转运蛋白1 乳腺癌组织 临床意义 乳腺浸润性导管癌 GLUT-1 乳腺纤维腺病 乳腺纤维腺瘤 正常乳腺组织 

分 类 号：R686[医药卫生—骨科学]