内脂素基因过表达对胰岛素抵抗大鼠胰岛素敏感性的影响  

Effects of visfatin gene overexpression on insulin sensitivity in the insulin-resistant rats induced by high-fat diet

作　　者：孙勤[1] 李伶[2] 杨刚毅[1] 李仁哲[2] 李钶[1] 齐晓亚[1] 李生兵[1] 陈文雯[1] 刘桦 唐毅 Guenther Boden 

机构地区：[1]重庆医科大学附属第二医院内分泌科和脂质研究中心,400010 [2]检验系临床生化教研室和教育部省部共建重点实验室 [3]Department of Pediatrics, University of Mississippi Medical Center, 2500 North State Street, Jackson, Mississippim, MS 39216-4505, USA [4]Oncology Ressearch. Centocor. Inc., Malvern. Pennsylvania, PA19355, USA [5]The Division of Endocrinology/Diabetes/Metabolism and the Clinical Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania, PA19140, USA

出　　处：《中华医学杂志》2008年第6期365-368,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目（30771037）;国家教委春晖计划基金资助项目（2003-56）;重庆市教委科研基金资助项目（KJ050304）;美国NIH基金资助项目（R01 HL073267）

摘　　要：目的探讨内脂素基因过表达对高脂诱导胰岛素抵抗（IR）大鼠胰岛素敏感性的影响。方法构建大鼠内脂素基因真核表达质粒，并用该质粒转染高脂喂养诱导的IR大鼠模型。在转染前后采用两次高胰岛素-正糖钳夹技术评价大鼠胰岛素敏感性的变化。结果成功构建了内脂素真核表达载体pcDNA3．1内脂素。实验组大鼠在pcDNA3．1内脂素质粒注射后3d血浆内脂素水平较注射前明显升高（2．19±0．36 vs 0．98±0．27，P〈0．01）；在两次胰岛素钳夹中，pcDNA3．1内脂素质粒注射后葡萄糖输注率较注射前明显升高[（32．6±1．2）mg·kg^-1·min^-1 vs （24．0±1．2）mg·kg^-1·min^-1，P〈0．01]；总胆固醇、高密度脂蛋白胆固醇则较质粒注射前明显降低[（2．36±0．22）mmol／L vs （1．60±0．21）mmol／L和（1．41±0．24）mmol／L vs（0．88±0．11）mmol／L，均P〈0．05]；在转染前后基础血糖和胰岛素水平无明显变化。结论内脂素质粒转染使IR大鼠血浆内脂素水平升高，胰岛素敏感性增强。Objective To investigate the effects of visfatin gene overexpression on insulin sensitivity in insulin-resistant （IR） rats induced by high-fat diet. Methods The recombinant visfatin plasmid was constructed and transfected into IR rats induced by high-fat diet.The euglycemic-hyperinsulinemic clamp experiments were performed for evaluation the change of insulin sensitivity before and after administration. Results The expression plasmid of visfatin were successfully constructed. After 3 days for plasmid injecting, plasma visfatin levels and glucose infusion rates were significantly increased（ 2. 19 ± 0. 36 vs 0. 98 ±0. 27 and 32. 6 ± 1.2 vs 24. 0 ± 1.2 mg·kg^-1·min^-1 , respectively, all P 〈0. 01 ）, and total cholesterol and high-density lipoprotein cholesterol （HDL-C） were significantly decreased（ 2. 36 ± 0. 22 vs 1.60 ± 0. 21 mmol/L and 1.41 ± 0. 24 vs 0. 88 ± 0. 11 mmol/L, respectively, all P 〈 0. 05 ） in high-fat diet rats. Conclusion The transfection of visfatin plasmid enhanced plasma visfatin level and improved insulin sensitivity in IR rats induced by high-fat diet.

关 键 词：内脂素 胰岛素抗体 正常血糖高胰岛素钳夹技术 

分 类 号：R686[医药卫生—骨科学]