FoxP3^＋调节性T细胞与慢性阻塞性肺疾病的相关性  

Correlation between FoxP3^＋ regulatory T cells and chronic obstructive pulmonary disease

作　　者：熊先智[1] 金阳[1] 周琼[1] 张晓菊[1] 杜雯 刘伟 黄士昂 

机构地区：[1]华中科技大学同济医学院附属协和医院呼吸科暨卫生部呼吸系疾病重点实验室,武汉430022 [2]湖北省干细胞中心

出　　处：《中华医学杂志》2008年第7期471-474,共4页National Medical Journal of China

摘　　要：目的研究慢性阻塞性肺疾病（COPD）急性发作期患者体内是否存在FoxP3^＋调节性T细胞（Treg）及其与COPD的关系。方法抽取21例COPD患者外周静脉血，用三色标记单抗流式细胞仪法，检测外周血中T细胞亚群CD4^＋CD25^＋，CD4^＋CD25^＋FoxP3^＋（CD4^＋Treg），CD8^＋CD25^＋，CD8^＋CD25^＋FoxP3^＋（CD8^＋Treg）的数量和百分比。用定量酶联免疫吸附法（EUSA）测定外周血中转化生长因子β1（TGF-β1），白细胞介素10（IL-10）和肿瘤坏死因子α（TNF-α）的浓度。取中等程度相关r≥0．3的结果进行分析讨论。结果在本组患者外周血中，CD4^＋CD25^＋为（18±6）％，CD4^＋Treg为（19±13）％，CD8^＋CD25^＋为（5±4）％，CD8^＋Treg为（12±10）％。直线相关分析结果表明，患者的年龄、病程、吸烟、白细胞总数和血pH都与Treg的数量或百分比呈显著相关，使其与患者的免疫构成复杂的因果关系。TGF-β1和1L-10在整体环境下与Treg无相关性。结论在COPD急性发作期患者外周血中有一定比例的CD4^＋Treg和CD8^＋Treg，对患者的免疫发挥明显的抑制作用，可以成为了解患者免疫状况的一组新指标。Objective To investigate whether FoxP3^＋ regulatory T cells （Treg） are present in the acute exacerbation of chronic obstructive pulmonary disease （AECOPD） and the correlation between Treg and COPD. Methods Peripheral blood samples were collected from 21 patients of AECOPD, 20 males and 1 female, aged （70±9） （52-85）. Lymphocytes were isolated by three-color labeled three colors monoclonal antibodies flow cytometry to examine the quantities and percentages of CD4^＋CD25^＋, CD4^＋CD25^＋ FoxP3^＋ （CD4^＋Treg）, CD8^＋ CD25^＋, and CD8^＋ CD25^＋ FoxP3^＋ （CD8^＋Treg）. ELISA was used to detect the expression of transforming growth factor β1 （TGF-β1）, interleukin-10 （IL-10）, and tumor necrosis factor α （TNF-α）. Middle correlation coefficient r≥0. 3 was analysed and discussed. Results The percentages of CD4^＋ CD25^＋, CD4^＋Treg, CD8^＋ CD25^＋, and CD8^＋ Treg in AECOPD were （18±6）%, （19 ±13）%, （5±4）%, and （12±10）% respectively. Linear correlation analysis indicated that the quantity and percentage of Treg were significantly correlated with age, course of disease, smoking index, quantity of white cells, and blood pH, and there were complex causal relations between the immunity of patients and these factors. However, TGF- β1 and IL-10 showed no correlation with Treg Conclusion CD4^＋ Treg and CD8^＋ Treg are expressed in the peripheral blood of AECOPD patients and contribute to the immune suppression of these patients, so they can be used as new markers of immunity of these patients.

关 键 词：肺疾病 阻塞性 T淋巴细胞 

分 类 号：R686[医药卫生—骨科学]