葛根素预处理对大鼠心肌缺血再灌注损伤保护作用及机制的实验研究  

Experimental Study of Protective Effects and Mechanism of Pueraria on Myocardial Ischemia Reperfusion Injury in Rats

作　　者：王建人[1] 尚立芝[1] 崔明霞[2] 李牧蔚[3] 

机构地区：[1]河南中医学院,河南郑州450008 [2]河南省胸科医院,河南郑州450008 [3]河南省人民医院,河南郑州450008

出　　处：《河南中医学院学报》2008年第1期32-34,共3页Journal of Henan University of Chinese Medicine

基　　金：河南中医学院苗圃工程(编号:MP2006-06)

摘　　要：目的:探讨葛根素对心肌缺血再灌注损伤的保护作用及机制。方法:60只SD大鼠被随机分为3组:假手术(sham)组、缺血再灌注损伤(IR)组、葛根素(PI)组。建立大鼠心肌缺血再灌注损伤模型,检测血清SOD、MDA、LDH和CK水平;观察心肌损伤的形态学改变。结果:与IR组比较,PI组SOD活力显著升高(P<0.05),MDA、LDH和CK含量显著降低(P<0.01),形态改变显著减轻(P<0.05)。结论:葛根素对缺血再灌注损伤的心肌有保护作用,其机制可能与增加SOD活性、稳定生物膜有关。Objective： To investigate the protective effects and its mechanism of pueraria on myocardial ischemia reperfusion injury （MIRI） in rats. Method： 60 SD rots were divided in to 3 groups randomly： sham operation group, ischemia reperfusion injury group （IR）, and pueraria injection （PI） group. The model of MIRI was produced, Serum activity of superoxide dismutase （SOD）, and contents of malondial dehyde （MDA）, lactate dehydrogenase （LDH）, creatine kinase （CK） were detected respectively, and the effects of pueraria on myocardial infarct sizes was estimated in rats with MIRI. Result： The activitie of SOD was significantly higher, the contents of MDA, LDH and CK were significantly lower, The myocardial infarct sizes （MIS） was significantly decreaser, in PI group than that in IR group. Conclusion： Pueraria can protect myocardial ischemia reperfusion injury in rats MIRI. The myocardial protective mechanism of pueraria may be realized by enchancing the activations of SOD, stabilizing myocardial cdltdar membrance.

关 键 词：葛根素 缺血再灌注损伤 CK 心肌梗死面积 

分 类 号：R285.5[医药卫生—中药学]