知黄复方抗心肌肥厚的处方筛选及抗小鼠心力衰竭作用考察  

Prescription bolting of total timosaponins with total astragalus saponins on myocardial hypertrophy and initial pharmacodynamic study of total timosaponins and total astragalus saponins complex

作　　者：王红[1] 秦文艳[1] 任雁[1] 陈瑛[1] 吴英良[1] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2008年第2期137-143,共7页Journal of Shenyang Pharmaceutical University

摘　　要：目的确定知黄复方中知母总皂苷与黄芪总皂苷合用抗心肌肥厚的最佳配比,通过概率和法证明处方的合理性;进行初步药效学试验。方法采用正交设计法。小鼠每天分早晚2次皮下注射盐酸异丙肾上腺素(isoprenaline)1 mg.kg-1,连续给药14 d造成心肌肥厚模型,测定通过正交配比;药效学试验采用知母总皂苷与黄芪总皂苷最佳剂量配比为(25+100)mg.kg-1组成知黄复方,用前述方法造模,14 d后分别于不同时间测定各组小鼠的心率、跑步时间、游泳时间、缺氧存活时间和心指数。结果从正交分析可知,知母总皂苷的极差(R)>黄芪总皂苷的极差,知母总皂苷与知母总皂苷+黄芪总皂苷组成的复方相比P>0.05,黄芪总皂苷与知母总皂苷+黄芪总皂苷组成的复方相比P<0.05;知母总皂苷与黄芪总皂苷合用剂量组的心指数最小,由金氏概率和法得到Q=1.17;知母总皂苷、黄芪总皂苷及知母总皂苷与黄芪总皂苷组成的复方均可减慢模型小鼠的心率;知母总皂苷与黄芪总皂苷组成的复方均可延长小鼠的跑步时间、游泳时间和缺氧存活时间,可显著降低模型小鼠心指数。结论黄芪总皂苷对盐酸异丙肾上腺素所致小鼠心肌肥厚具有显著的抑制作用;知母总皂苷与黄芪总皂苷在所选择剂量范围内的理论最佳配比为(25+100)mg.kg-1;知母总皂苷与黄芪总皂苷组成的复方有抗盐酸异丙肾上腺素所致小鼠心力衰竭作用。Objective To ascertain the optimal proportion of total timosaponins and total astragalus saponins which have effect on myocardial hypertrophy, to demonstrate the rationality of the prescription, and to investigate its initial pharmacodynamics. Methods The myocardial hypertrophy model was established by administrating the isoprenaline subcutaneously in mouse. The cardiac index was determined by using orthogohal-design method in order to definite the optimal proportion of total timosaponins and total astragalus saponins. In the pharmacodynamic experiment, the effect of the complex prescription, which comprised the two ingredients in the optimal proportion, was investigated by measuring heart rate, running time, swimming time, survival time in hypoxia and cardiac index after the model was set up as above. Results The study revealed that R total astragalus saponins〉Rtotal timosaponins; the cardiac index was found to be the minimum in the total timosaponins and total astragalus saponins （25 ＋ 100） mg· kg^-1 treated group. It was observed that there was antagonistic role against the increase of the heart rate in total timosaponins, total astragalus saponins, total timosaponins and total astragalus saponins treated group. The heart rate, running time, swimming time and survival time was found to be increased in the total timosaponins, total timosaponins and total astragalus saponins treated groups. It also revealed the antagonistic effect on myocardial hypertrophy pronouncedly in total timosaponins and total astragalus saponins treated group. Conclusions Total timosaponins has the restrain effect on myocardial hypertrophy induced by isoprenaline in mouse. Among the selected doses, the theoretical optimal proportion of total timosaponins and total astragalus saponins is （25 ＋ 100）mg· kg^-1. The complex prescription can enhance the action.

关 键 词：知母总皂苷 黄芪总皂苷 心肌肥厚 心力衰竭 

分 类 号：R96[医药卫生—药理学]