MiniFiler^(TM)试剂盒在LCN-STR分型中的应用  

The application of MiniFiler^(TM) kit in LCN-STR genotyping

作　　者：吴微微[1] 郑小婷[1] 郝宏蕾[1] 陈勇 

机构地区：[1]浙江省公安厅刑侦总队,浙江杭州310009 [2]义乌市公安局,浙江义乌322000

出　　处：《中国法医学杂志》2008年第1期23-25,共3页Chinese Journal of Forensic Medicine

摘　　要：目的评估MiniFilerTM试剂盒在LCN-STR分型中的法医学应用价值。方法采用MiniFilerTM与IdentifilerTM试剂盒,对49份常量与39份LCN检材,包括血迹、精斑、骨骼等7类常见检材的检验结果进行比较,并对两种试剂盒的检测灵敏度进行比较。结果在49份常量检材的检验结果中,两种试剂盒的STR分型结果全部一致;在39份LCN生物检材的检验结果中,MiniFilerTM试剂盒获得全部STR分型的有30份,部分分型的5份,阴性的4份;IdentifilerTM试剂盒获得部分STR分型的22份,阴性的17份。MiniFilerTM试剂盒检验成功率明显高于IdentifilerTM试剂盒;MiniFilerTM试剂盒的检测灵敏略高于IdentifilerTM。结论MiniFilerTM试剂盒可显著提高LCN生物检材的检验成功率,适合应用于法庭科学实际检案中LCN生物检材的检验鉴定。Objective To evaluate the application value of MiniFiler^TM kit in LCN-STR genotyping in forensic science. Methods Compared the testing results of MiniFiler^TM and Identifiler^TM kits in 49 general quantity biological samples and 39 LCN-biological samples, including blood stains, sperm stains, bones, and so on. Meanwhile the sensitivity of these two kits were also compared. Results Full concordance between Identifiler^TM and MiniFiler^TM kits was observed in all of 49 general quantity biological samples. For 39 LCN-biological samples, only 22 samples could be genotyped in partial loci and 17 samples negative with the Identifiler^TM kit, while 30 samples could be genotyped in all loci, 5 samples in partial loci and 4 samples negative with the MiniFiler^TM kit. Therefore, there was a higher success rate for LCN-biological samples typing with MiniFiler^TM kit than with Identifiler^TM kit. In addition, the sensitivity of the MiniFiler^TM kit was also a little higer than the Identifiler^TM kit. Conclusion The results demonstrated that the MiniFiler^TM kit can markedly improve the typing success rate of LCN-biological samples, and is suitable for analyzing difficult biological samples in forensic practice .

关 键 词：法医物证学 低拷贝模板 Mini-STR 灵敏度 

分 类 号：D919[医药卫生—法医学]