嗜水气单胞菌分离株的鉴定与菌株肠毒素及致病性研究  

Identification of clinically isolated Aeromonas hydrphila and investigation of pathogenicity of its interoxin detected.

作　　者：李刚山[1] 范泉水[1] 徐庆[2] 周卫国[1] 李作生[1] 张富强[1] 邱薇[1] 张琳[1] 王承珠[1] 袁贵红[1] 

机构地区：[1]成都军区疾病预防控制中心军事医学研究所,云南昆明650032 [2]昆明市疾病预防控制中心,云南昆明650034

出　　处：《中国热带医学》2008年第3期374-375,共2页China Tropical Medicine

摘　　要：目的对嗜水气单胞菌进行鉴定并进行肠毒素检测与致病性分析。方法采用常规法分离培养,电子显微镜观察形态,用法国梅里埃VITEK32全自动微生物鉴定仪进行系统鉴定。乳鼠灌胃法测定热稳定性肠毒素(ST),小白鼠腹腔注射法测定其毒力。结果检出的嗜水气单胞菌均产热稳定性肠毒素(ST),具备较强的致病性。结论分离的嗜水气单胞菌具有较强毒力,与致病性密切相关。Objective To identify the isloates of Aeromonas hydrophila clinically isolated and investigate the phathogenicity of its enterotoxin. Methods The isolates were cultured and observed under electron microscope and systemically identified with automatic bioassay device. The pathogenicity of enterotoxin was investigated by peritoneally injecting the enterotoxin into mouse. Results The Aeromonas hydrophila producing thermostatic enterotoxin is highly pathogenic. Conclusion Aeromonas hydrophila is associated with diarrhea.

关 键 词：腹泻 嗜水气单胞菌 毒力 热稳定性肠毒素 

分 类 号：R378.99[医药卫生—病原生物学]