3,3',5,5'-四甲基联苯胺-H_2O_2-辣根过氧化物酶伏安酶联免疫分析法测定人血清免疫球蛋白E  

3,3',5,5'-Tetramethylbenzidine(TMB)-H_2O_2-HRP Voltammetric Immunoassay for the Determination of IgE in Human Serum

作　　者：何亚楠[1] 陈洪渊[1] 

机构地区：[1]南京大学化学化工学院,南京210093

出　　处：《高等学校化学学报》1997年第8期1306-1308,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：A differential pulse voltammetric immunoassay associated with the TMB-H2O2-HRP for the determination of IgE antibody was developed. The enzymatic reaction product produced by HRP catalyzing the oxidation of H2O2 with TMB exhibits a sensitive voltammet-ric response at 0.10V(vs.Ag/AgCl) in pH4.0 phosphate-citrate buffer solution. The de-tection limit of the present method for IgE antibody was 4.0IU/mL, which was four times lower than that obtained by traditional spectrophotometric ELISA procedure. It has been ap-plied to the determination of IgE in human serum with satisfactory results.A differential pulse voltammetric immunoassay associated with the TMB-H2O2-HRP for the determination of IgE antibody was developed. The enzymatic reaction product produced by HRP catalyzing the oxidation of H2O2 with TMB exhibits a sensitive voltammet-ric response at 0.10V(vs.Ag/AgCl) in pH4.0 phosphate-citrate buffer solution. The de-tection limit of the present method for IgE antibody was 4.0IU/mL, which was four times lower than that obtained by traditional spectrophotometric ELISA procedure. It has been ap-plied to the determination of IgE in human serum with satisfactory results.

关 键 词：TMB IGE HRP 免疫分析 ELISA 示差脉冲伏安法 

分 类 号：Q512.2[生物学—生物化学] R446.62[医药卫生—诊断学]