水通道蛋白1在哮喘豚鼠嗜酸粒细胞迁移趋化中的作用  

Role of aquaporin 1 in the migration of eosinophils from asthmatic guinea pigs

作　　者：雷霏[1] 朱冬冬[1] 王心蕊[2] 关桂梅[1] 姜云鹏[3] 董震[1] 

机构地区：[1]吉林大学中日联谊医院耳鼻咽喉头颈外科,长春130033 [2]吉林大学人兽共患病研究所教育部人兽共患病重点实验室 [3]吉林大学基础医学院病理生物学教育部重点实验室

出　　处：《中华耳鼻咽喉头颈外科杂志》2008年第2期130-133,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的观察豚鼠哮喘模型中水通道蛋白1（aquaporin 1，AQP1）在嗜酸粒细胞（eosinophils，EOS）运动中的作用，探讨AQP1是否可以作为EOS趋化活性的标志。方法选取健康雄性Hartley系豚鼠建立哮喘模型，分别提取外周血及支气管肺泡灌洗液中的EOS，应用原位分子杂交和免疫荧光及激光扫描共聚焦显微镜技术，从基因水平及蛋白质水平检测外周血及支气管肺泡灌洗液中EOS表达AQP1的情况。结果AQP1在哮喘豚鼠外周血和支气管肺泡灌洗液的EOS内均有表达，外周血中AQP1 mRNA（平均灰度值92．904±3．290，x^-±s，下同）和蛋白（荧光强度425．081±17．474）的表达强度均高于灌洗液中AQP1 mRNA（109．200±5．756）和蛋白（279．926±11．293）的表达强度，差异具有统计学意义（t值分别为9．519和27．020，P值均〈0．05）。结论变应性疾病状态下，EOS可能通过合成更多的AQP1来加速其向炎性靶器官的运动，AQP1有望成为EOS趋化活性的标志之一。Objective To investigate the role of aquaporin 1 （AQP1） in the migration of eosinophils （EOS） and to determine if AQP-1 can be viewed as the chemotactic activity marker of EOS. Methods Asthma model of guinea pigs were developed and EOS were purified from both peripheral blood and bronchoalveolar lavage fluid （BALF） . The smears of EOS were studied by in situ hybridization for determining AQP1 mRNA and immunofluorescence under laser scanning confocal microscope for determining AQP1 protein. Results AQP1 was found expressed in EOS both from peripheral blood and from BALF. Compared with the expression of AQP1 mRNA （ mean grey value 109. 200 ± 5.756, x^- ± s ） and protein （ average fluorescence intensity 279. 926 ± 11. 293 ） in EOS from BALF, there was stronger expression of AQP1 mRNA （92. 904 ± 3. 290 ） and protein （425.081 ± 17. 474 ） in EOS from peripheral blood. The difference beth of AQP1 mRNA （ t = 9. 519, P 〈 0.05 ） and protein （ t = 27.020, P 〈 0. 05 ） were considered statistically significant respectively. Conclusions AQP1 plays a crucial role in EOS movement. It is possible that EOS produce more AQP1 protein to accelerate its migration to inflammatory tiusse under allergic disease and EOS with AQP1 highly expressed are activated. AQP1 can be viewed as the chemotactic activity marker of EOS.

关 键 词：模型 动物 哮喘 水通道蛋白1 嗜酸细胞 

分 类 号：R686[医药卫生—骨科学]