去势加地塞米松肌肉注射法兔骨质疏松模型建立的实验研究  

Study on establishment of rabbit osteoporosis model by ovariectomy plus dexamethasone intramuscular injection

作　　者：张堃[1] 孟国林[1] 刘建[1] 袁志[1] 张钦[1] 王军[1] 李晓娟[1] 张志敏[1] 李生[1] 

机构地区：[1]第四军医大学西京医院骨科,西安710032

出　　处：《中国骨质疏松杂志》2008年第2期69-72,共4页Chinese Journal of Osteoporosis

基　　金：陕西省国际科技合作重点项目(2005KW-14)

摘　　要：目的探究应用去势加肌肉注射地塞米松的方法建立兔骨质疏松模型的可行性。方法将24只5月龄雌性新西兰兔随机分成假手术+肌注生理盐水组、去势+肌注生理盐水组和去势+肌注地塞米松组,每组8只动物。后两组动物行双侧卵巢切除术。术前和术后2、3月时采用双能X线吸收骨密度测量仪及术后3月应用micro-CT进行测量分析。结果micro-CT三维重建分析表明,去势加肌注地塞米松肌肉注射组兔术后3月时股骨远端BMD、BMC和骨小梁立体测量的一些指标与假手术的差值有统计学意义,去势+肌注地塞米松组和去势组兔骨小梁稀疏、断裂、排列紊乱,但前者更为明显。结论去势+肌注地塞米松法建立新西兰兔骨质疏松症模型切实可行,造模时间较单纯去势法更短,3个月即可。Objective To investigate the feasibility of the establishment of rabbit osteoporosis model by ovariectomy plus Dexamethasone intramuscular injection. Methods 24 five months old female New Zealand white rabbits were divided into three groups equally. Group A, shame operation plus Sodium Chloride intramuscular injection. Group B, ovariectomy plus Sodium Chloride intramuscular injection. Group C, ovariectomy plus Dexamethasone intramuscular injection. The bilateral ovaries of the last two groups were ectomized. 2 and 3 months before and after operation, the BMD was measured by double-energy X-ray absorption meter, respectively. 3 months after operation, micro-CT was used to detect the experimental rabbits. Results The micro-CT reconstruction analysis showed that 3 months after operation, the difference of BMD, BMC and trabecula stereology parameter of distal femur has statistical value between group 1 and 3. The trabecular of the distal femur in group 2 and group 3 all became raritas, broken and disordered and it was more obvious with group 3. Conclusions These results suggest that ovariectomy plus Dexamethasone intramuscular injection is a good method to establish New Zealand rabbit osteoporosis model. Three months are enough to establish the osteoporosis model using ovariectomy plus dexamethasone intramuscular infection, shorter than using only ovariectomy.

关 键 词：去势 地塞米松 骨质疏松症 动物模型 显微CT 

分 类 号：R965[医药卫生—药理学] R589.5[医药卫生—药学]